无中文名称
| 中文名称 | 无中文名称 |
|---|---|
| 中文同义词 | |
| 英文名称 | EAD1 HCl |
| 英文同义词 | EAD1 HCl;EAD1(free base);1,2-Ethanediamine, N1-[2-[[[1-[(4-chlorophenyl)methyl]-1H-1,2,3-triazol-4-yl]methyl]amino]ethyl]-N2-(7-chloro-4-quinolinyl)-N1-methyl- |
| CAS号 | 1644388-26-0 |
| 分子式 | C24H27Cl2N7 |
| 分子量 | 484.42 |
| EINECS号 | |
| 相关类别 | |
| Mol文件 | 1644388-26-0.mol |
| 结构式 |  |
无中文名称 性质
| 沸点 | 687.5±65.0 °C(Predicted) |
|---|---|
| 密度 | 1.31±0.1 g/cm3(Predicted) |
| 酸度系数(pKa) | 8.41±0.50(Predicted) |
EAD1 是一种有效的自噬 (autophagy) 抑制剂，在肺癌和胰腺癌细胞中具有抗增殖活性。EAD1 还诱导凋亡 (apoptosis)。
EAD1 (0-200 μM; 72 hours) has antiproliferative activity against human cancer cell lines.
EAD1 (5-75 μM; 24 hours) induces apoptosis in the H460 cells in a concentration-dependent manner.
EAD1 (1-25 μM; 24 hour) increases the punctate LC3 signal in H460 cells.
Cell Proliferation Assay
| Cell Line: | H460 and HCC827 nonsmall cell lung cancer (NSCLC) and BxPC3 pancreatic cancer cells |
| Concentration: | 0, 50, 100, 150, 200 μM |
| Incubation Time: | 72 hours |
| Result: | IC 50 s of 11, 7.6, and 5.8 μM for H460, HCC827, and BxPC3 cells, respectively. |
Apoptosis Analysis
| Cell Line: | H460 cells |
| Concentration: | 5, 25, 50, and 75 μM |
| Incubation Time: | 24 hours |
| Result: | Induction of apoptosis. |
Western Blot Analysis
| Cell Line: | H460 cells |
| Concentration: | 1, 2.5, 5, 10, 25 μM |
| Incubation Time: | 24 hours |
| Result: | Caused concentration and time-dependent increases in LC3-II levels. |