| Company Name: |
Sigma-Aldrich
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021-61415566 800-8193336 |
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orderCN@merckgroup.com |
| Products Intro: |
Product Name:Anti-Luciferase antibody, Mouse monoclonal
Purity:clone LUC-1, purified from hybridoma cell culture Package:.2ML Remarks:L2164-.2ML
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| | Anti-Luciferase antibody, Mouse monoclonal Basic information |
| | Anti-Luciferase antibody, Mouse monoclonal Chemical Properties |
| storage temp. | -20°C | | form | buffered aqueous solution |
| | Anti-Luciferase antibody, Mouse monoclonal Usage And Synthesis |
| Uses | Anti-Luciferase antibody, Mouse monoclonal has been used in:
- immunoblotting
- immunocytochemistry
- immunohistochemistry
| | General Description | Anti-Luciferase antibody, Mouse monoclonal , (mouse IgG1 isotype) is derived from the LUC-1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with firefly (Photinus pyralis) luciferase. Luciferase, a reporter protein contains two binding pockets, one pocket for ATP and other one for luciferin substrate. Both the pockets are in close proximity to each other. | | Biochem/physiol Actions | Luciferase catalyzes a bioluminescent reaction in the presence of substrate luciferin as well as Mg2+, oxygen and ATP. The luciferase assay is rapid, sensitive, and unlike the CAT assay, does not require a radioactive substrate. Anti-Luciferase antibody can detect the luciferase enzyme expression in situ, providing a means to study the cellular localization of the signal sequence activation. |
| | Anti-Luciferase antibody, Mouse monoclonal Preparation Products And Raw materials |
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