ChemicalBook > Product Catalog >Food Additives >Enzyme >EC 3.4.21.14

EC 3.4.21.14

EC 3.4.21.14 Suppliers list
Company Name: Hebei Yanxi Chemical Co., Ltd.
Tel: +8617531190177
Email: peter@yan-xi.com
Products Intro: Product Name:PROTEASE ALKALINE
CAS:9014-01-1
Purity:0.99 Package:1kg;20USD|100kg;10USD|1000kg;1USD Remarks:Factory direct sales
Company Name: Henan Bao Enluo International TradeCo.,LTD
Tel: +86-17331933971 +86-17331933971
Email: deasea125996@gmail.com
Products Intro: Product Name:EC 3.4.21.14
CAS:9014-01-1
Purity:99% Package:1kg;10USD
Company Name: Henan Tianfu Chemical Co.,Ltd.
Tel: +86-0371-55170693 +86-19937530512
Email: info@tianfuchem.com
Products Intro: Product Name:Alkaline Protease
CAS:9014-01-1
Purity:99% Package:25KG;5KG;1KG
Company Name: Hangzhou FandaChem Co.,Ltd.
Tel: 008657128800458; +8615858145714
Email: fandachem@gmail.com
Products Intro: Product Name:Alkaline Protease fandachem
CAS:9014-01-1
Purity:As coa Package:As request Remarks:9014-01-1
Company Name: career henan chemical co
Tel: +86-0371-86658258
Email: sales@coreychem.com
Products Intro: Product Name:EC 3.4.21.14
CAS:9014-01-1
Purity:99% Package:1kg;1USD

EC 3.4.21.14 manufacturers

  • PROTEASE ALKALINE
  • PROTEASE ALKALINE pictures
  • $20.00 / 1kg
  • 2023-11-15
  • CAS:9014-01-1
  • Min. Order: 1kg
  • Purity: 0.99
  • Supply Ability: 20 tons
  • PROTEASE ALKALINE
  • PROTEASE ALKALINE pictures
  • $0.00 / 1KG
  • 2023-09-06
  • CAS:9014-01-1
  • Min. Order: 1KG
  • Purity: 99%
  • Supply Ability: 500000kg
  • EC 3.4.21.14
  • EC 3.4.21.14 pictures
  • $10.00/ kg
  • 2023-08-02
  • CAS:9014-01-1
  • Min. Order: 1kg
  • Purity: 99%
  • Supply Ability: 500t/month
EC 3.4.21.14 Basic information
Defination
Product Name:EC 3.4.21.14
Synonyms:protease for total dietary fiber assay;protease type viii bacterial;protease type xxxi;Proteinase, bacterial,Alkaline Protease, Protease from Bacillus licheniformis, Proteinase from Bacillus licheniformis, Subtilisin Carlsberg, Subtilo peptidase A;ALCALASE2.4LFG;ALCALASE2.5L;Subtilisin Carslberg;Alcalase? 2.4L
CAS:9014-01-1
MF:Unspecified
MW:0
EINECS:232-752-2
Product Categories:25kg/drum;Hydrolases;Specialty Enzymes
Mol File:Mol File
EC 3.4.21.14 Structure
EC 3.4.21.14 Chemical Properties
density 1.3
vapor pressure 0Pa at 25℃
storage temp. 2-8°C
form powder
color white
Water Solubility 100g/L at 25℃
Stability:store cold
LogP-3.1 at 25℃
CAS DataBase Reference9014-01-1
EPA Substance Registry SystemSubtilisin (9014-01-1)
Safety Information
Hazard Codes Xn,N
Risk Statements 37/38-41-42-36/37/38-50-22
Safety Statements 23-24-26-36/37/39-22-36/37-61-45-39
RIDADR 3082
WGK Germany 2
RTECS UK9540000
3-10
HS Code 35079090
Hazardous Substances Data9014-01-1(Hazardous Substances Data)
ToxicityLD50 orl-rat: 3700 mg/kg FCTXAV 7,581,69
MSDS Information
EC 3.4.21.14 Usage And Synthesis
DefinationProteases (EC 3.4.21.62) belong to the class of enzymes known as hydrolases, which catalyze hydrolysis of various bonds in presence of water. Proteases are also referred to as Peptidases or Proteinases. Proteases catalyze proteolysis of peptide bonds in polypeptides, proteins and selective hydrolysis of carboxylic esters and amino esters. There are different classes of Proteases, i.e. serine, threonine, cysteine, aspartate, glutamic acid and metallo – proteases.
	EC 3.4.21.14
Chemical PropertiesThese are proteolytic enzymes which take the form of light-colored, free-flowing powders. A protein containing numerous amino acids
UsesThis is a proteolytic enzyme isolated from the fermentation of Bacillus licheniformis. It is a serine endoproteinase with a broad specificity towards native and denatured proteins, and is active under alkaline conditions. It is for use in Total Dietary Fiber Assays (TDF-100A).
UsesProtease from Bacillus sp. has been used to determine its proteolytic activity by spectrophotometric method.
UsesProtease from Bacillus licheniformis has been used to digest the neurolemma in the antennal lobe of the moth; Heliothis virescens.
General DescriptionProteolytic enzymes are known to possess catalytic, non-catalytic and ancillary domains. Proteases are broadly classified as endopeptidases and exopeptidases. Functionally they are divided as aspartic, glutamic, cysteine, threonine, serine and metalloproteases.
Biochem/physiol ActionsProtease catabolizes proteins by hydrolysis of peptide bonds. Proteases are inactivated by serine active-site inhibitors, such as phenylmethylsulfonyl fluoride (PMSF) and diisopropylfluorophosphate. Proteases, secreted from Bacillus sp., typically have molecular weights ranging from 20,000 to 30,000. They are typcially stabilized by Ca2+ and have high isoelectric points.
Safety ProfileModerately toxic by ingestion. Aneye irritant. When heated to decomposition it emits toxicfumes of NOx.
Potential ExposureThese commercial proteolytic enzymes are used in laundry detergent formulations
Purification MethodsThis alkaline protease is purified 211-fold by affinity chromatography using 4-(4-aminophenylazo)phenylarsonic acid complex to activated CH-Sepharose 4B. It is inhibited by 2-phenylethane boronic acid, PMSF, 3,4-dichloroisocoumarin, acetone and benzamide. [Chandraskaren & Dhar Anal Biochem 150 141 1985, Schomburg & Schomburg Springer Handbook of Enzymes 2nd Edn vol 7 p 286 2002.] Synexin (from bovine liver) M 47,000 Da. This Ca binding protein is purified by (NH4)2SO4 precipitation, then by a specific pH step elution from a chromatofocusing medium in the absence of ampholytes. The pI is 7.5. [Scott et al. Anal Biochem 149 163 1985.]
IncompatibilitiesIncompatible with oxidizers (chlorates, nitrates, peroxides, permanganates, perchlorates, chlorine, bromine, fluorine, etc.); contact may cause fires or explosions. Keep away from alkaline materials, strong bases, strong acids, oxoacids, epoxides.
EC 3.4.21.14 Preparation Products And Raw materials
Tag:EC 3.4.21.14(9014-01-1) Related Product Information
N-BUTYLISOCYANIDE METHYL ISOCYANOACETATE PHENYLSELENOL TRIS(2,2,6,6-TETRAMETHYL-3,5-HEPTANEDIONATO)EUROPIUM(III) 2,4-PENTANEDIONE, SILVER DERIVATIVE COBALT(II) ACETYLACETONATE DICHLORO(ETHYLENEDIAMINE)PLATINUM(II) Ethyl isocyanoacetate SALCOMINE Benzyl isocyanide Ferric acetylacetonate TRIS(2,2,6,6-TETRAMETHYL-3,5-HEPTANEDIONATO)DYSPROSIUM(III) Tosylmethyl isocyanide Aluminum acetylacetonate 1,1,3,3-TETRAMETHYLBUTYL ISOCYANIDE Cupric acetylacetonate TERT-BUTYL ISOCYANIDE EC 3.4.21.14