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CARBOXYPEPTIDASE A

CARBOXYPEPTIDASE A Suppliers list
Company Name: Henan Tianfu Chemical Co.,Ltd.
Tel: +86-0371-55170693 +86-19937530512
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Products Intro: Product Name:Carboxypeptidase A
CAS:11075-17-5
Purity:99% Package:25KG;5KG;1KG
Company Name: Hubei Jusheng Technology Co.,Ltd.
Tel: 18871490254
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Products Intro: Product Name:CARBOXYPEPTIDASE A
CAS:11075-17-5
Purity:0.99 Package:5KG;1KG
Company Name: Creative Enzymes
Tel: 1-516-855-7709
Email: info@creative-enzymes.com
Products Intro: Product Name:carboxypeptidase A
CAS:11075-17-5
Remarks:A zinc enzyme formed from procarboxypeptidase A. Isolated from cattle, pig and dogfish pancreas, and other sources including mast cells and skeletal muscle. Type example of peptidase family M14.
Company Name: Career Henan Chemica Co
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Products Intro: Product Name:CARBOXYPEPTIDASE A
CAS:11075-17-5
Purity:0.99 Package:1KG;7KG Judy@corechem.com
Company Name: Shanghai Acmec Biochemical Technology Co., Ltd.
Tel: +undefined18621343501
Email: product@acmec-e.com
Products Intro: Product Name:Carboxypeptidase A
CAS:11075-17-5
Purity:>=20 units/mg protein Package:500U
CARBOXYPEPTIDASE A Basic information
Product Name:CARBOXYPEPTIDASE A
Synonyms:Carboxypolypeptidase, Peptidyl-L-amino-acid hydrolase from bovine pancreas;Carboxypeptidase A from bovine pancreas,Carboxypolypeptidase, Peptidyl-L-amino-acid hydrolase;Peptidyl-L-amino-acid hydrolase from bovine pancreas;Carboxypeptidase A?Agarose;E.C. 3.4.12.2;E.C. 3.4.2.1;PEPTIDYL-L-AMINO-ACID HYDROLASE;PEPTIDYL-L-AMINO-ACID HYDROLASE TYPE II-PMSF
CAS:11075-17-5
MF:NULL
MW:0
EINECS:234-297-5
Product Categories:
Mol File:Mol File
CARBOXYPEPTIDASE A Structure
CARBOXYPEPTIDASE A Chemical Properties
density 1.00 g/mL at 20 °C
Fp 4 °C
storage temp. 2-8°C
form suspension
color white
CAS DataBase Reference11075-17-5
Safety Information
Hazard Codes F,Xn,B,Xi
Risk Statements 11-38-42-48/20-63-65-67-36-36/37/38
Safety Statements 36/37-45-62-36-26-23-16-24-22
RIDADR UN 1294 3/PG 2
WGK Germany 2
10
MSDS Information
ProviderLanguage
SigmaAldrich English
CARBOXYPEPTIDASE A Usage And Synthesis
Chemical PropertiesSuspension (white)
UsesThe enzyme from Sigma has been used as a comparison to study the specificity of Metarhizium anisopliae carboxypeptidase A (MeCPA). MeCPA had been genetically engineered to facilitate the removal of polyhistidine tags from the C-termini of recombinant proteins. It has also been used to de-tyrosinate α-tubulin, in vitro, in order to induce high affinity to ethyl-N-phenylcarbamate (EPC) sepharose.
DefinitionAn enzyme that catalyzes the cleavage of amino acids from a protein at the C-terminus of a polypeptide chain.
General DescriptionCarboxypeptidase A-agarose product is prepared by the immobilization of carboxypeptidase A, originally isolated from the bovine pancreas, to activated 4% crosslinked beaded agarose.
Industrial usesCarboxypeptidase A(CPA) is an enzyme of the digestive system that is known to cleave amino acids favouring the C-terminal end as well as certain esters. This enzymatic activity depends on the metal at the catalytic site. Zn2+ and some Co2+-containing CPAs exhibit peptidase function, whilst esterase function has been seen by CPAs containing a variety of divalent d-block metals. CPA has a size similar to CA, consisting of about 300 amino acids and a molecular mass of 34 kDa.
Biochem/physiol ActionsCarboxypeptidase as isolated from bovine pancreas glands is a metalloenzyme that contains 1 g atom of zinc per mole of protein. It catalyzes the hydrolysis of the carboxyl-terminal peptide bond in peptides and proteins. It is primarily specific to aromatic and hydrophobic side chains such as phenylalanine, tryptophan or leucine. The enzyme also exhibits esterase activity. It is inhibited by beta-phenylpropionate and indole acetate.
Purification MethodsCarboxypeptidase A is purified by DEAE-cellulose chromatography, activation with trypsin and dialysed against 0.1M NaCl, yielding crystals. It is recrystallised by dissolving in 20 mL of M NaCl and dialysed for 24hours each against the following salts present in 500mL of 0.02M sodium veronal pH 8.0, 0.5M NaCl, 0.2M NaCl and 0.15M NaCl. The last dialysate usually induces crystallisation. If it does not crystallise, then dialyse the last solution against 0.02M sodium veronal containing 0.10M NaCl. Only 2 or 3 recrystallisations are required to attain maximum activity. [Cox et al. Biochemistry 3 44 1964.] Enzyme activity is measured by hydrolysing hippuryl-L-phenylalanine (or phenylacetic acid) and observing the rate of change of optical density at 254nm (reaction extinction coefficient is ~0.592 cm2/Wmole at pH 7.5) [Bergmyer Methods in Enzymatic Analysis (Academic Press) 1 436 1974].
CARBOXYPEPTIDASE A Preparation Products And Raw materials
Tag:CARBOXYPEPTIDASE A(11075-17-5) Related Product Information
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