PI-PLC, 1-Phosphatidyl-D-myo-inositol inositol phosphohydrolase, cyclic-phosphate forming

PI-PLC, 1-Phosphatidyl-D-myo-inositol inositol phosphohydrolase, cyclic-phosphate forming Suppliers list
Company Name: Creative Enzymes
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Products Intro: Product Name:phosphatidylinositol diacylglycerol-lyase
CAS:37288-19-0
Remarks:This enzyme is bacterial. Activity is also found in animals, but this activity is due to the presence of EC 3.1.4.11, phosphoinositide phospholipase C.
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Products Intro: Product Name:Phospholipase C, Phosphatidylinositol-specific from Bacillus cereus
CAS:37288-19-0
Purity:BR Package:1mg; 10mg; 100mg; 1G; 10G
Company Name: Sigma-Aldrich  
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Products Intro: Product Name:Phospholipase C, Phosphatidylinositol-specific
CAS:37288-19-0
Purity:buffered aqueous glycerol solution, main >= 1,000units/mg pr Package:5units, 25units Remarks:P8804
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Products Intro: Product Name:PhospholipaseC,Phosphatidylinositolspecific,fromBacilluscereus
CAS:37288-19-0
Purity:>=1,000units/mgprotein Package:5U
Company Name: Guangzhou Tomums Life Science Co., Ltd.  
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Products Intro: Product Name:Phospholipase C, Phosphatidylinositol-specific from Bacillus cereus
CAS:37288-19-0
Package:5UN;25UN
PI-PLC, 1-Phosphatidyl-D-myo-inositol inositol phosphohydrolase, cyclic-phosphate forming Basic information
Product Name:PI-PLC, 1-Phosphatidyl-D-myo-inositol inositol phosphohydrolase, cyclic-phosphate forming
Synonyms:PC-PLC;PI-PLC, 1-Phosphatidyl-D-myo-inositol inositol phosphohydrolase, cyclic-phosphate forming;phosphatidylinositol diacylglycerol-lyase;Phospholipase C, Phosphatidylinositol-specific from Bacillus cereus lyophilized powder, >=1,000 units/mg protein (Lowry)
CAS:37288-19-0
MF:
MW:0
EINECS:232-638-2
Product Categories:
Mol File:Mol File
PI-PLC,  1-Phosphatidyl-D-myo-inositol  inositol  phosphohydrolase,  cyclic-phosphate  forming Structure
PI-PLC, 1-Phosphatidyl-D-myo-inositol inositol phosphohydrolase, cyclic-phosphate forming Chemical Properties
storage temp. -20°C
form lyophilized powder
Safety Information
WGK Germany 3
RTECS SZ6116000
MSDS Information
PI-PLC, 1-Phosphatidyl-D-myo-inositol inositol phosphohydrolase, cyclic-phosphate forming Usage And Synthesis
UsesPhospholipase C, Phosphatidylinositol-specific from Bacillus cereus has been used:
  • in the hydrolysis of substrates p-nitrophenylphosphorylcholine (p-NPPC) and p-nitrophenylphosphorylphosphate (p-NPP)
  • to cleave glycosylphosphatidylinositol (GPI) anchor of lynx1 protein and its detachment from plasma membrane
  • to cleave immunolabeled HeLa cells

UsesPhospholipase C, Phosphatidylinositol-specific from Bacillus cereus has been used to cleave GPI-anchored protein from green fluorescent protein- glycosylphosphatidylinositol (GFP-GPI) from the cell membrane. It has also been used in invasion assay to test the involvement of PLAP in T. cruzi invasion to human epithelial type 2 (HEp-2) cells.
General DescriptionPhosphatidylinositol-specific phospholipase C (PI-PLC) from Bacillus cereus is a 298 residual protein. It belongs to the class of ubiquitous enzymes. PI-PLC is characterized with a single globular domain and it folds into eight (βα) barrel sheets, out of which only six βα units are found in barrel, lacking helices between strands IV and V and V and VI.
Biochem/physiol ActionsUsed for the release of GPI anchored proteins from the membrane.
PI-PLC, 1-Phosphatidyl-D-myo-inositol inositol phosphohydrolase, cyclic-phosphate forming Preparation Products And Raw materials
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