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| | 5,5′-Dithiobis(2-nitrobenzoic acid) Basic information |
| | 5,5′-Dithiobis(2-nitrobenzoic acid) Chemical Properties |
| Melting point | 240-245 °C (dec.)(lit.) | | Boiling point | 671.9±55.0 °C(Predicted) | | density | 1.67 | | refractive index | 1.6900 (estimate) | | storage temp. | Keep in dark place,Sealed in dry,Room Temperature | | pka | 1.57±0.25(Predicted) | | form | Yellow solid | | color | Pale Yellow to Light Yellow | | Water Solubility | Soluble in water and ethanol. | | Merck | 14,3551 | | BRN | 1896821 | | Stability: | Stable. Incompatible with strong bases, strong oxidizing agents. | | InChIKey | KIUMMUBSPKGMOY-UHFFFAOYSA-N | | CAS DataBase Reference | 69-78-3(CAS DataBase Reference) | | EPA Substance Registry System | Benzoic acid, 3,3'-dithiobis[6-nitro- (69-78-3) |
| Hazard Codes | Xi | | Risk Statements | 36/37/38 | | Safety Statements | 26-36 | | WGK Germany | 3 | | RTECS | DG9650000 | | F | 10 | | TSCA | Yes | | HS Code | 29309070 |
| | 5,5′-Dithiobis(2-nitrobenzoic acid) Usage And Synthesis |
| Chemical Properties | Crystalline | | Uses | Reagent for determination of sufhydryl groups.5,5'-dithiobis-(2-nitrobenzoic acid) is widely utilized as a non-fluorescent probe to quantify the number or the concentration of thiol groups in a sample. It acts as a cleavable cross-linking agent. It finds an important application as a sensitive reagent for determining free sulfhydryl content in proteins, peptides, and tissues. It is also employed in high-performance liquid chromatography (HPLC) to determine the alkylthiol content. | | Uses | 5,5′-Dithiobis(2-nitrobenzoic acid) is a water soluble reagent mainly used for the derivatization of sulfhydryl groups. | | Uses | 5,5′-Dithiobis(2-nitrobenzoic acid) is an membrane-impermeant thiol/disulfide exchange blocker. It is also used to quantify the number or concentration of thiol groups in a sample. | | Definition | ChEBI: An organic disulfide that results from the formal oxidative dimerisation of 2-nitro-5-thiobenzoic acid. An indicator used to quantify the number or concentration of thiol groups. | | General Description | 5,5′-Dithiobis(2-nitrobenzoic acid) is also called DTNB or Ellman′s reagent. It is a water soluble reagent, which interacts with thiol groups, and can be used conveniently at neutral pH. Thiolate anion (RS-) reacts with Ellman′s reagent (DTNB2-), to produce 2-nitro-5-thiobenzoate anion (TNB2-) and one mixed disulfide (R-S-TNB-). TNB2- has a high molar absorption coefficient, and absorbs at 412nm. This absorption is independent of pH, at pH> 7.3. |
| | 5,5′-Dithiobis(2-nitrobenzoic acid) Preparation Products And Raw materials |
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