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GLUCOSE-6-PHOSPHATE DEHYDROGENASE

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Company Name: Henan DaKen Chemical CO.,LTD.
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Products Intro: Product Name:GLUCOSE-6-PHOSPHATE DEHYDROGENASE
CAS:9001-40-5
Purity:99% Package:100g,500g,1kg,5kg,10kg
Company Name: Henan Tianfu Chemical Co.,Ltd.
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Products Intro: CAS:9001-40-5
Purity:99% Package:500G;1KG;5KG;25KG
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Products Intro: Product Name:G-6-PD
CAS:9001-40-5
Purity:150u/mg solid Package:1KU
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Products Intro: Product Name:GLUCOSE-6-PHOSPHATE DEHYDROGENASE
CAS:9001-40-5
Purity:99% Package:1KG;1USD
Company Name: Hubei xin bonus chemical co. LTD
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Products Intro: Product Name:GLUCOSE-6-PHOSPHATE DEHYDROGENASE
CAS:9001-40-5
Purity:0.99 Package:5KG;1KG

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GLUCOSE-6-PHOSPHATE DEHYDROGENASE Basic information
Product Name:GLUCOSE-6-PHOSPHATE DEHYDROGENASE
Synonyms:glucose-6-phosphate dehydrogenase from baker's yeast (s. cerevisiae);Glucose -6-phosphoric acid dehydrogenase;ZWISCHENFERMENT;ZWISCHENFERMENT G6P-DH;ZWISCHENFERMENT TYPE IX;ZWISCHENFERMENT TYPE V;ZWISCHENFERMENT TYPE VII;ZWISCHENFERMENT TYPE XV
CAS:9001-40-5
MF:n.a.
MW:0
EINECS:232-602-6
Product Categories:enzyme;Metabolomics;1.1.x.x Acting on hydroxyl groups;1.x.x.x Oxidoreductases;Carbohydrate MetabolismEnzyme Class Index;General Metabolic Enzymes
Mol File:Mol File
GLUCOSE-6-PHOSPHATE DEHYDROGENASE Structure
GLUCOSE-6-PHOSPHATE DEHYDROGENASE Chemical Properties
Melting point ~120℃
storage temp. -20°C
form suspension
color white
Water Solubility Soluble in water and dilute buffer.
EPA Substance Registry SystemDehydrogenase, glucose 6-phosphate (9001-40-5)
Safety Information
Hazard Codes Xi
Risk Statements 36/37/38
Safety Statements 26-36-24/25
WGK Germany 3
3-10
TSCA Yes
HS Code 35079090
MSDS Information
ProviderLanguage
SigmaAldrich English
GLUCOSE-6-PHOSPHATE DEHYDROGENASE Usage And Synthesis
Chemical PropertiesWhite to yellow powder
Purification MethodsThe enzyme is useful for measuring pyridine nucleotides in enzyme recycling. The enzyme from Baker's yeast has been purified by (NH4)2SO4 fractionation, Me2CO precipitation, a second (NH4)2SO4 fractionation, concentration by DEAE-SF chromatography, a third (NH4)2SO4 fractionation and recrystallisation. Crystallisation is induced by addition of its coenzyme NADP, which in its presence causes rapid separation of crystals at (NH4)2SO4 concentration much below that required to precipitate the amorphous enzyme. To recrystallise, the crystals are dissolved in 0.01M NADP (pH 7.3) with (NH4)2SO4 at 0.55 saturation, and the crystals appear within 10 to 60minutes. After standing for 2-3days (at 4o) the (NH4)2SO4 is increased to 0.60 of saturation, and more than 80% of the activity in the original crystals is recovered in the fresh crystals. [Noltmann et al. J Biol Chem 236 1255 1961]. Large amounts can be obtained from rat livers. The livers are extracted with 0.025M phosphate buffer (pH 7.5) and precipitated with 3M (NH4)2SO4 (70% of activity). The precipitate is dissolved in 3volumes of 0.025M phosphate (pH 7.5), dialysed against this buffer + 0.2mM EDTA at 4o for 5hours, then diluted to 1% protein and the nucleic acids are precipitated by addition of 0.4volumes of 1% protamine sulfate. (NH4)2SO4 is added to a concentration of 2M (pH adjusted to 7.0 with NH3), the precipitate is discarded and the supernatant is adjusted to 2.8M (NH4)2SO4, dialysed, and the protein is adjusted to 1% and treated with Ca3(PO4)2 gel. The gel is added in three steps (1.5mL of 0.4% gel/mL per step), and the gel is removed by centrifugation after each addition. The third gel adsorbed 50% of the activity. The gel is eluted with 0.2M phosphate buffer (pH 7.4, 40mL/g of gel; 60% recovery). The extract is precipitated in 3volumes of (NH4)2SO4 (adjusted to 4M) to give enzyme with an activity of 30Wmoles/mg of protein per hour. [Lowry et al. J Biol Chem 236 2746 1961.] The Km values for the yeast enzyme are 20WM for G-6P and 2WM for NADP (Tris pH 8.0, 10-2 M MgCl2, 38o) [Noltmann & Kuby The Enzymes VII 223 1963].
GLUCOSE-6-PHOSPHATE DEHYDROGENASE Preparation Products And Raw materials
Raw materialsRibonucleic acid-->CELLULOSE PHOSPHATE
Tag:GLUCOSE-6-PHOSPHATE DEHYDROGENASE(9001-40-5) Related Product Information
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