| Company Name: |
Sigma-Aldrich
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021-61415566 800-8193336 |
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orderCN@merckgroup.com |
| Products Intro: |
Product Name:Pyruvate Kinase (PK)
Purity:from rabbit muscle Package:1EA Remarks:10109045001
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| | Pyruvate Kinase (PK) Basic information |
| | Pyruvate Kinase (PK) Chemical Properties |
| storage temp. | 2-8°C | | form | suspension |
| | Pyruvate Kinase (PK) Usage And Synthesis |
| Uses | Pyruvate Kinase (PK) has been used in the kinase assay. | | Uses | Pyruvate kinase has been used to measure ATPase activity and in the determination of adenylate concentration. | | General Description | ATP:pyruvate 2-O-phosphotransferase
Pyruvate kinase has a molar mass of 237,000 and exists as a tetramer. Each polypeptide chain of this tetramer has a molar mass of 57,200. The enzyme contains two identical catalytic particles called protomers. Each of these protomers contains two polypeptide chains. Each protomer contains one site each for Mn2+ and phosphoenolpyruvate. | | Biochem/physiol Actions | Pyruvate kinase catalyzes the irreversible conversion of P-enolpyruvate and ADP to pyruvate and ATP with the utilization of a proton. The first step is the transfer of phosphate group from P-enolpyruvate to ADP with the formation of bound enolate of pyruvate and ATP. In the second step, a proton is added to enolate to generate the keto form of pyruvate. Apart from this, the enzyme exhibits other activities, such as ATP- and bicarbonate-dependent ATPase, phosphorylation of fluoride and hydroxylamine, ATP-dependent phosphorylation of glycolate, and decarboxylation of oxaloacetate. |
| | Pyruvate Kinase (PK) Preparation Products And Raw materials |
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