| Company Name: |
Absin Bioscience Inc.
|
| Tel: |
021-38015121 18438616290 |
| Email: |
lanwu@univ-bio.com |
| Products Intro: |
Product Name:Trypsin-EDTA Solution
|
| Company Name: |
Merck KGaA
|
| Tel: |
21-20338288 |
| Email: |
ordercn@merckgroup.com |
| Products Intro: |
Product Name:Trypsin-EDTA solution
|
|
| | TRYPSIN-EDTA SOLUTION Basic information |
| | TRYPSIN-EDTA SOLUTION Chemical Properties |
| storage temp. | -20°C | | form | solution | | PH | 7.0-9.0 | | biological source | Porcine pancreas |
| | TRYPSIN-EDTA SOLUTION Usage And Synthesis |
| Uses | The typical use for this product is in removing adherent cells from a culture surface. The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the culture. It can be used with endothelial cell cultures. | | Biochem/physiol Actions | Trypsin cleaves peptides on the C-terminal side of lysine and arginine residues. The rate of hydrolysis of this reaction is slowed if an acidic residue is on either side of the cleavage site and hydrolysis is stopped if a proline residue is on the carboxyl side of the cleavage site. The optimal pH for trypsin activity is 7-9. Trypsin can also act to cleave ester and amide linkages of synthetic derivatives of amino acids. EDTA is added to trypsin solutions as a chelating agent that neutralizes calcium and magnesium ions that obscure the peptide bonds on which trypsin acts. Removing these ions increases the enzymatic activity. Serine protease inhibitors, including DFP, TLCK, APMSF, AEBSEF, and aprotinin, amongst others, will inhibit Trypsin. |
| | TRYPSIN-EDTA SOLUTION Preparation Products And Raw materials |
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