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| | 4-METHYLUMBELLIFERYL-N-ACETYL-BETA-D-GLUCOSAMINIDE Basic information |
| | 4-METHYLUMBELLIFERYL-N-ACETYL-BETA-D-GLUCOSAMINIDE Chemical Properties |
| | 4-METHYLUMBELLIFERYL-N-ACETYL-BETA-D-GLUCOSAMINIDE Usage And Synthesis |
| Chemical Properties | 4-METHYLUMBELLIFERYL-N-ACETYL-BETA-D-GLUCOSAMINIDE is White Crystalline Solid | | Uses | 4-METHYLUMBELLIFERYL-N-ACETYL-BETA-D-GLUCOSAMINIDE is used for the assay of b-Acetylglucosaminidase. A fluorogenic substrate used for the diagnosis of GM2 gangliosidoses in leukocytes. Genetic defects in human b-hexosaminidase isozy mes (Hex A & Hex B) result in various clinical and biochemical types of GM2 gangliosidosis. They include Tay Sachs disease, Sandhoff disease and other forms. Fluorescence: max. Abs. l = 325nm, max. Em. l = 378nm | | Uses | 4-Methylumbelliferyl N-acetyl-β-D-glucosaminide has been used as a substrate for assaying
- β-N-acetylglucosaminidase (NAGase) from soil samples
- chitinase from Fusarium oxysporum using fluorimetric assay
- β-N-acetylhexosaminidases from Arabidopsis thaliana roots and leaves samples
| | General Description | 4-Methylumbelliferyl N-acetyl-β-D-glucosaminide (4-MUF-NAG) contains chitin monomer, N-acetyl-D-glucosamine (NAG) and a 4-methylumbelliferone (7-hydroxy-4-methylcoumarin) (4-MUF) moiety. | | Biochem/physiol Actions | 4-Methylumbelliferyl N-acetyl-β-D-glucosaminide (4-MUF-NAG) is a synthetic fluorogenic substrate for N-acetyl-β-D-glucosaminidase (NAGase). The enzyme functionality is assessed by its ability to hydrolyze the N-acetyl-D-glucosamine (NAG) residue in 4-MUF-NAG. 4-MUF-NAG is also a substrate for Chitinase and N-acetylhexosaminidase. |
| | 4-METHYLUMBELLIFERYL-N-ACETYL-BETA-D-GLUCOSAMINIDE Preparation Products And Raw materials |
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