4,4'-(dithiodicarbonothioyl)dimorpholine
| 中文名称 | 4,4'-(dithiodicarbonothioyl)dimorpholine |
|---|---|
| 中文同义词 | 化合物JX06;双(吗啉硫代羰基)二硫化物;双(吗啉代硫代羰基)二硫化物(JX 06);双(吗啉噻吩羰基)二硫化物;化合物JX06,10 MM DMSO 溶液;双(吗啉硫代羰基)二硫化物/JX06;JX06 试剂;JX06 ,S0983 |
| 英文名称 | 4,4'-(dithiodicarbonothioyl)dimorpholine |
| 英文同义词 | 4,4'-(dithiodicarbonothioyl)dimorpholine;Morpholine, 4,4-(dithiodicarbonothioyl)bis-;Bis(morpholinocarbonothioyl) persulfide;JX 06;Bis(morpholinothiocarbonyl) disulfide;Apoptosis,JX06,JX-06,Inhibitor,PDH kinase,inhibit,Pyruvate dehydrogenase kinase,JX 06,PDHK;JX06, 10 mM in DMSO;DI(4-MORPHOLINYL)DITHIOPEROXYANHYDRIDE |
| CAS号 | 729-46-4 |
| 分子式 | C10H16N2O2S4 |
| 分子量 | 324.51 |
| EINECS号 | 211-980-6 |
| 相关类别 | |
| Mol文件 | 729-46-4.mol |
| 结构式 |  |
4,4'-(dithiodicarbonothioyl)dimorpholine 性质
| 熔点 | 136-137 °C |
|---|---|
| 沸点 | 466.3±55.0 °C(Predicted) |
| 密度 | 1.4302 (rough estimate) |
| 折射率 | 1.5650 (estimate) |
| 储存条件 | Store at -20°C |
| 溶解度 | 溶于二甲基亚砜 |
| 形态 | 固体 |
| 酸度系数(pKa) | 0.58±0.20(Predicted) |
| 颜色 | 白色至米白色 |
| EPA化学物质信息 | Morpholine, 4,4'-(dithiodicarbonothioyl)bis- (729-46-4) |
| Target | Value |
|
PDK1
(Cell-free assay) | 0.049 μM |
|
PDK2
(Cell-free assay) | 0.101 μM |
|
PDK3
(Cell-free assay) | 0.313 μM |
JX06 barely shows inhibitory activity against PDK4 at a concentration of 10 μM.
JX06 (1-10 μM; 48 hours) induces cell apoptosis in cancer cells with high ECAR/OCR.
JX06 (0-0.6 μM; 72 hours) dose-dependently suppresses the growth of A549 cells.
JX06 (0.1-10 μM; 6-24 hours) inhibits PDHA1 phosphorylation in A549 cells in a time- and dose-dependent manner.
JX06 (1-10 μM) increases glucose uptake and intracellular ATP level and reduces aerobic glycolysis determined by the lactate production in A549 cells.
JX06 (1-10 μM; 24 hours) induces ROS generation in cancer cells with high extracellular acidification rate (ECAR)/ oxygen consumption rate (OCR) .
Apoptosis Analysis
| Cell Line: | A549, EBC-1, HT-29 and H460 cells |
| Concentration: | 0, 1, 3, 10 μM |
| Incubation Time: | 48 hours |
| Result: | Induces cell apoptosis in A549 and EBC-1 cells. |
Cell Viability Assay
| Cell Line: | A549 cells |
| Concentration: | 0, 0.2, 0.4, 0.6 μM |
| Incubation Time: | 72 hours |
| Result: | Inhibits the viability of A549 cells in a dose dependent manner. |
Western Blot Analysis
| Cell Line: | A549 cells |
| Concentration: | 0, 0.1, 0.3, 1, 3, 10 μM |
| Incubation Time: | 0, 6, 12, 24 hours |
| Result: | Decreased PDHA1 phosphorylation at both serine 293 and serine 232 (S293 and S232) in a time- and dose-dependent manner. |
JX06 (40-80 mg/kg; i.p. for 21 days) inhibits tumor growth in vivo.
| Animal Model: | A549 subcutaneous xenograft mice |
| Dosage: | 40, 80 mg/kg |
| Administration: | I.p. injections for 21 days |
| Result: |
Reduced tumor weights and 67.5% tumor volume at the dose of 80 mg/kg compared with the vehicle control.
Well tolerated at the administration dose. |
安全信息
| TSCA | TSCA listed |
|---|