黄柏苷
| 中文名称 | 黄柏苷 |
|---|---|
| 中文同义词 | 二氢黄柏苷;黄柏甙;(二氢)黄柏甙;黄柏苷 |
| 英文名称 | phellamurin |
| 英文同义词 | phellamurin;(2R)-7-(β-D-Glucopyranosyloxy)-2,3-dihydro-3β,5-dihydroxy-2α-(4-hydroxyphenyl)-8-(3-methyl-2-butenyl)-4H-1-benzopyran-4-one;(dihydro) Phellodendron;4H-1-Benzopyran-4-one,7-(b-D-glucopyranosyloxy)-2,3-dihydro-3,5-dihydroxy-2-(4-hydroxyphenyl)-8-(3-methyl-2-buten-1-yl)-,(2R,3R)-;4H-1-Benzopyran-4-one, 7-(β-D-glucopyranosyloxy)-2,3-dihydro-3,5-dihydroxy-2-(4-hydroxyphenyl)-8-(3-methyl-2-buten-1-yl)-, (2R,3R)-;(2R,3R)-3,5-dihydroxy-2-(4-hydroxyphenyl)-8-(3-methylbut-2-enyl)-7-[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy-2,3-dihydrochromen-4-one;Cluster of differentiation 243,intestinal,anti-tumor,inhibit,MDR1,Pgp,CD243,glycoside,p-AKT,p-mTOR,p-PI3K,Multidrug resistance protein 1,P-glycoprotein,P-gp,ABCB1,Apoptosis,Flavonone,Phellamurin,Inhibitor |
| CAS号 | 52589-11-4 |
| 分子式 | C26H30O11 |
| 分子量 | 518.52 |
| EINECS号 | |
| 相关类别 | 标准品;植物提取物;标准品 -对照药材;黄酮 |
| Mol文件 | 52589-11-4.mol |
| 结构式 |  |
黄柏苷 性质
| 沸点 | 861.5±65.0 °C(Predicted) |
|---|---|
| 密度 | 1.504±0.06 g/cm3(Predicted) |
| 溶解度 | 溶于氯仿、二氯甲烷、乙酸乙酯、DMSO、丙酮等。 |
| 形态 | 粉末 |
| 酸度系数(pKa) | 7.15±0.60(Predicted) |
Phellamurin (0-10 μg/mL; 48 hours; U2OS and Saos-2 cells) treatment leads to a repression of cell viability in U2OS and Saos-2 cells in a dose-dependent manner.
Phellamurin (0-10 μg/mL; 48 hours; U2OS and Saos-2 cells) treatment concentration-dependently promots the apoptosis of U2OS and Saos-2 cells.
Phellamurin (0-10 μg/mL; 48 hours; U2OS and Saos-2 cells) treatment declines the ratios of p-PI3K/PI3K, p-AKT/AKT, and p-mTOR/mTOR in U2OS and Saos-2 cells.
Cell Viability Assay
| Cell Line: | U2OS and Saos-2 cells |
| Concentration: | 0 μg/mL, 2.5 μg/mL, 5 μg/mL, and 10 μg/mL |
| Incubation Time: | 48 hours |
| Result: | Suppressed the viability of U2OS and Saos-2 cells in a concentration-dependent manner. |
Apoptosis Analysis
| Cell Line: | U2OS and Saos-2 cells |
| Concentration: | 0 μg/mL, 2.5 μg/mL, 5 μg/mL, and 10 μg/mL |
| Incubation Time: | 48 hours |
| Result: | Induced apoptosis of U2OS and Saos-2 cells in a concentration-dependent manner. |
Western Blot Analysis
| Cell Line: | U2OS and Saos-2 cells |
| Concentration: | 0 μg/mL and 10 μg/mL |
| Incubation Time: | 48 hours |
| Result: | Repressed the PI3K/AKT/mTOR pathway in U2OS and Saos-2 cells. |
Phellamurin (50 mg/kg/day; intraperitoneal injection; daily; for 21 days; female BALB/c nude mice) treatment represses osteosarcoma tumor growth in vivo. The ratios of p-PI3K/PI3K, p-AKT/AKT, and p-mTOR/mTOR are decreased in xenograft in Phellamurin-treated mice.
| Animal Model: | Athymic female BALB/c nude mice (6 weeks old ) injected with U2OS cells |
| Dosage: | 50 mg/kg/day |
| Administration: | Intraperitoneal injection; daily; for 21 days |
| Result: | Repressed osteosarcoma tumor growth in vivo. |