| Company Name: |
Sigma-Aldrich
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| Tel: |
021-61415566 800-8193336 |
| Email: |
orderCN@merckgroup.com |
| Products Intro: |
Product Name:Phytohemagglutinin-L (PHA-L)
Purity:>95% (SDS-PAGE) Package:1EA Remarks:11249738001
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| | PhytoheMagglutinin-L (PHA-L) Basic information |
| | PhytoheMagglutinin-L (PHA-L) Chemical Properties |
| storage temp. | 2-8°C | | form | lyophilized (clear, colorless solution after reconstitution) |
| | PhytoheMagglutinin-L (PHA-L) Usage And Synthesis |
| Uses | Phytohemagglutinin-L (PHA-L) consists of only L-type subunits (isolectin L4, "leuko-agglutinin"), and is well suited for high-efficiency induction and the functional analysis of T-lymphocytes. It is highly purified and tested for high-efficiency stimulation of human lymphocytes. It is also used as positive controls for peripheral blood mononuclear cells (PBMC) stimulation. | | General Description | PHA-L is purified from Phaseolus vulgaris (red kidney bean) by standard chromatographic techniques.
Phytohemagglutinin (PHA), the lectin extract from the red kidney bean, consists of only L-type subunits (isolectin L4, "leuko-agglutinin"). It is an N-acetylgalactosamine/galactose sugar-specific lectin. PHA consists of five isolectins (L4E0, L3E1, L2E2, L1E3, L0E4) each being a tetramer held together by noncovalent forces. The subunits L (leukocyte reactive) have a high affinity for lymphocyte surface receptors, but little for those of erythrocytes, and are responsible for the mitogenic properties of the isolectins. E (erythrocyte reactive) is responsible for the erythrocyte-agglutinating properties. | | Biochem/physiol Actions | Phytohemagglutinin (PHA) has mitogenic and potent cell agglutinating activities. It binds to T-cell membranes and stimulates cell division and metabolic activity. PHA has the ability to stimulate close contacts between cell membranes. |
| | PhytoheMagglutinin-L (PHA-L) Preparation Products And Raw materials |
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