| Company Name: |
Merck Millipore |
| Tel: |
1-400-889-1988 400-889-1988 |
| Email: |
bioscience@merckgroup.com |
| Company Name: |
Merck KGaA |
| Tel: |
21-20338288 |
| Email: |
ordercn@merckgroup.com |
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| | FAS LIGAND PLUS(TM), HUMAN Basic information |
| Product Name: | FAS LIGAND PLUS(TM), HUMAN | | Synonyms: | FAS LIGAND PLUS, HUMAN, RECOMBINANT;FAS LIGAND PLUS(TM), HUMAN;FAS LIGAND PLUS, HUMAN | | CAS: | | | MF: | | | MW: | 0 | | EINECS: | | | Product Categories: | | | Mol File: | Mol File | ![FAS LIGAND PLUS(TM), HUMAN Structure]() |
| | FAS LIGAND PLUS(TM), HUMAN Chemical Properties |
| storage temp. | -20C | | form | Lyophilized |
| WGK Germany | WGK 1 | | Storage Class | 11 - Combustible Solids |
| | FAS LIGAND PLUS(TM), HUMAN Usage And Synthesis |
| Uses | Recombinant, human Fas ligand (amino acids 103-281) fused to a 26-amino acid linker and FLAG®-tag at the N-terminus and expressed in HEK293 cells. Glycosylation of recombinant human sFasL is similar or identical to natural human FasL. The molecular weight is ~32 kDa (nonglycosylated) and ~37-42 kDa (glycosylated) by SDS-PAGE under reducing conditions. The recombinant protein is produced in HEK293 cells. It does not require enhancer for activity. Useful for cytotoxicity assays.
Fas Ligand (FasL/APO-1L/CD95L) is a ~37-42 kDa type II transmembrane protein belonging to the TNF family. Interaction between FasL and Fas induces apoptosis of Fas-sensitive cells. FasL is implicated in CTL-mediated-killing, activation-induced cell death, creation of immune-privileged sites and tissue homeostasis. The extracellular domain of human FasL can be cleaved off by a metalloprotease, generating soluble FasL (sFasL). Circulating sFasL can be detected in serum of patients with melanoma and certain types of lymphoma. | | Biological Activity | ≥1 ng/ml kills Fas-sensitive cells |
| | FAS LIGAND PLUS(TM), HUMAN Preparation Products And Raw materials |
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