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DL-Dithiothreitol

DL-Dithiothreitol Suppliers list
Company Name: Yunbio Tech Co.,Ltd.
Tel: 010-60605551
Email: yunbiochem@126.com
Products Intro: Product Name:DL-1,4-Dithiothreitol (DTT)
CAS:3483-12-3
Purity:98% Package:10g;100g;500g;1kg;5kg;10kg,100kg,1ton Remarks:Carbohydrates
Company Name: Casorganics US Corp
Tel: +17326109938
Email: sales@casorganics.com
Products Intro: Product Name:DL-1,4-Dithiothreitol
CAS:3483--12-3
Purity:>99% Package:1KG;USD|5KG;USD|25KG;USD
Company Name: Henan DaKen Chemical CO.,LTD.
Tel: +86-371-55531817
Email: info@dakenchem.com
Products Intro: Product Name:DL-1,4-Dithiothreitol
CAS:3483-12-3
Purity:99% Package:100g,500g,1KG,10KG,100KG
Company Name: Henan Tianfu Chemical Co.,Ltd.
Tel: 0371-55170693
Email: info@tianfuchem.com
Products Intro: Product Name:3483-12-3
CAS:3483-12-3
Purity:0.99 Package:25KG,5KG;1KG;500G
Company Name: Shanghai Zheyan Biotech Co., Ltd.
Tel: 18017610038
Email: zheyansh@163.com
Products Intro: Product Name:DTTDL-1,4-Dithiothreitol
CAS:3483-12-3
Purity:BR,99% Package:5g;25g;100g

Lastest Price from DL-Dithiothreitol manufacturers

  • DL-1,4-Dithiothreitol
  • US $1000.00 / KG
  • 2019-07-06
  • CAS:3483-12-3
  • Min. Order: 25KG
  • Purity: 98%
  • Supply Ability: 10tons
  • DL-1,4-Dithiothreitol
  • US $1000.00 / KG
  • 2019-07-06
  • CAS:3483-12-3
  • Min. Order: 25KG
  • Purity: 98%
  • Supply Ability: 10tons
DL-Dithiothreitol Basic information
Common reductant Uses
Product Name:DL-Dithiothreitol
Synonyms:DL-DITHIOTHREITOL;4-dimercapto-3-butanediod-threo-1;Cleland regent;D-Dtt;d-threo-1,4-dimercapto-2,3-butanediol;D-threo-1,4-Dimercapto-2,3-butanediol,;Sputolysin;Threitol, 1,4-dithio-
CAS:3483-12-3
MF:C4H10O2S2
MW:154.25
EINECS:222-468-7
Product Categories:APIS;Cleland's Reagent;Chemistry;Protein Modification Reagents;Life Science Chemical Reagents;proteinmod
Mol File:3483-12-3.mol
DL-Dithiothreitol Structure
DL-Dithiothreitol Chemical Properties
Melting point 41-44 °C(lit.)
alpha -0.2~+0.2°(20℃/D)(c=5,H2O)
Boiling point 125 °C
density 1.04 g/mL at 20 °C
vapor density 5.3 (vs air)
refractive index 1.5200 (estimate)
Fp >230 °F
storage temp. 2-8°C
solubility H2O: 50 mg/mL, clear, colorless
pkapK1:8.9 (25°C)
form Powder
color White
OdorUnpleasant Odor
PH4.0-6.0 (20-25℃, 0.1m in H2O)
PH Range4 - 6 at 15,4 g/l at 25 °C
Water Solubility freely soluble
λmaxλ: 260 nm Amax: 0.400
λ: 280 nm Amax: 0.100
Sensitive Air Sensitive
Merck 14,3376
BRN 1719757
Stability:Stability Stable, but heat sensitive. Incompatible with strong oxidizing agents. Keep frozen at -20 to -10 C.
InChIKeyVHJLVAABSRFDPM-UHFFFAOYSA-N
CAS DataBase Reference3483-12-3(CAS DataBase Reference)
NIST Chemistry Reference2,3-Butanediol, 1,4-dimercapto-, (r*,r*)-(3483-12-3)
EPA Substance Registry System1,4-Dithiothreitol (3483-12-3)
Safety Information
Hazard Codes Xn
Risk Statements 25-36/37/38-22-20/21/22
Safety Statements 26-45-37/39-36
RIDADR UN 3335
WGK Germany 3
RTECS EK1610000
3-10-16-23
TSCA Yes
HS Code 29309099
MSDS Information
ProviderLanguage
SigmaAldrich English
ACROS English
ALFA English
DL-Dithiothreitol Usage And Synthesis
Common reductantDL-1, 4-DL-1, 4-Dithiothreitol is a common reducing agent, also known as DTT with a strong reduction capability. Its reduction capability is largely due to the conformational stability of the six-membered ring (containing disulfide bone) of its oxidation state. The reducing capability is affected by the pH value and also takes effect only in the case of a pH being greater than 7. This is because it is only the deprotonated thiolate anion (-S-) that is actively in reduction reaction while thiol (an-SH) is not; and the pKa of mercapto group is typically around~8.3.
The reduction of the disulfide bond mediated by DL-1, 4-Dithiothreitol mainly consists of a two-step continuous sulfhydryl-disulfide exchange: wherein the intermediate state formed by the first step of the reaction is very unstable because the secondary sulfhydryl group of DTT tends to connected by the oxidized sulfur atom, making the intermediate state be rapidly converted to the cyclic oxidation structure of DTT, thus completing the reduction of disulfide bonds.
Since being easily oxidized by air, the stability of DL-1, 4-Dithiothreitol (DTT) is relatively poor; frozen storage or processing in inert gas can extend its duration life. Owing to the low nucleophilicity of the protonated sulfur, with the decreased pH value, the effective reducing capability of DTT is also reduced; Moreover, Tris (2-carboxyethyl) phosphine HCl (TCEP hydrochloride) can be used as the substitute of DTT at low pH value and is also more stable than DTT.
DL-1, 4-Dithiothreitol has an antioxidant property that can protect the reductive groups on the enzyme molecules and maintain a reducing environment and stabilize the enzyme activity. DTT also contain certain RNase inhibitors which can inhibit the enzymatic activity of RNase. It has a similar effect as mercaptoethanol (b-mercaptoethanol) but the irritating odor and toxicity of DTT is much smaller and lower than the toxicity of mercaptoethanol. Moreover, DTT has a similar effect as mercaptoethanol even at a concentration of being seven times lower, but the price of DTT is slightly higher. It can be used as the protein thiol group protecting agent and the cleavage of the protein disulfide bond as well as sequence analysis.
Current implemented law regarding the poisons and toxic materials has designated β-mercaptoethanol and reagent-containing β-mercaptoethanol as poison. DL-1, 4-Dithiothreitol has similar effect as β-mercaptoethanol and can protect the SH group of protein, cutting the disulfide bond and therefore will be gradually paid attention by related applications.
The above information is edited by the chemicalbook of Dai Xiongfeng.
UsesMolecular Biology
Chemical PropertiesWhite crystalline powder
UsesDithiothreitol is a redox reagent commonly used as a reducing agent for thiolated DNA. Dithiothreitol is also used to reduce the disulfide bonds of proteins.
UsesDL-dithiothreitol (DTT) is a sulfhydryl compound that acts both as a reagent reducing disulfide bonds and as a protein denaturant on staphylococcal biofilm (Wu et al. 2011). Thanks to these properties, DTT is routinely used in clinical microbiology to liquefy respiratory specimens, but it has also been proven effective to detach biofilm from orthopaedic prosthesis (Drago et al. 2012, 2013). Since it is crucial to discriminate implant-related infections from aseptic loosening in orthopaedics, and because of the difficulty to diagnose subclinical infections, a prompt diagnosis of indwelling device-related infection is important for successful treatments (Borens et al. 2013). A fast microbiological diagnosis and bacterial identification should be recommended in order to set up a specific antimicrobial therapy. According to this strategy, the bacterial detachment from biofilm on explants should be accelerated by supporting the pathogen viability.
DefinitionChEBI: The threo-diastereomer of 1,4-dimercaptobutane-2,3-diol.
ApplicationDL-Dithiothreitol (DTT) is a chemical debonding technique that also has the goal of driving bacte-ria out of biofilms. A potential advantage of DTT compared with sonication is that tissue can also be evaluated without implants. Drago and col-leagues compared DTT with sonication in patients with prosthetic joint infection or aseptic loosening. Sensitivity was better with DTT (85.7%) versus sonication (71.4%), whereas both had identical specificity (94.1%). DTT had better sensitivity and specificity compared with standard tissue culture. A recent study supports the potential cost benefits of both DTT and son-ication compared with standard culture.
Biological ActivityReagent for maintaining SH groups in the reduced state.
Tag:DL-Dithiothreitol(3483-12-3) Related Product Information
L-(+)-THREOSE L-THREITOL 1,4-BUTANEDITHIOL L-DITHIOTHREITOL DL-1,4-DITHIOTHREITOL (D10) 2,3-Butanediol Sodium thiosulfate Panthenol Mercaptoacetic acid DL-Dithiothreitol Sorbitol DL-Dithiothreitol Succimer 2-Ethylhexyl mercaptoacetate Thioacetamide Maltitol 1,4-Butanediol 2,2'-Thiodiethanol