| Company Name: |
GL Biochem (Shanghai) Ltd
|
| Tel: |
21-61263452 13641803416 |
| Email: |
ymbetter@glbiochem.com |
| Products Intro: |
Product Name:Z-Leu-Arg-AMC
CAS:156192-32-4
Purity:95% HPLC,98% HPLC Package:10mg, 25mg, 50mg, 100mg, 500mg, 1g,10g
|
|
| | Z-LEU-ARG-7-AMINO-4-METHYLCOUMARIN Basic information |
| Product Name: | Z-LEU-ARG-7-AMINO-4-METHYLCOUMARIN | | Synonyms: | Z-LEU-ARG-7-AMINO-4-METHYLCOUMARIN;Z-LEU-ARG-AMC;Z-LEU-ARG-AMC HYDROCHLORIDE;L-Argininamide, N-[(phenylmethoxy)carbonyl]-L-leucyl-N-(4-methyl-2-oxo-2H-1-benzopyran-7-yl)-;Benzyloxycarbonyl-L-leucyl-L-arginine 4-methylcoumaryl-7-amide;CBZ-Leu-Arg-AMC hydrochloride salt | | CAS: | 156192-32-4 | | MF: | C30H38N6O6 | | MW: | 578.66 | | EINECS: | | | Product Categories: | | | Mol File: | 156192-32-4.mol |  |
| | Z-LEU-ARG-7-AMINO-4-METHYLCOUMARIN Chemical Properties |
| density | 1.31±0.1 g/cm3(Predicted) | | storage temp. | Store at 0-5°C | | solubility | Soluble in DMSO | | form | solid | | pka | 11.13±0.46(Predicted) | | color | White | | Stability: | Stable for 2 years from date of purchase as supplied. Solutions in DMSO may be stored at -20°C for up to 3 months. Protect from light! |
| | Z-LEU-ARG-7-AMINO-4-METHYLCOUMARIN Usage And Synthesis |
| Description | Z-Leu-Arg-AMC (156192-32-4) is a sensitive Cathepsin K fluorogenic substrate (Km=8μM, kcat/Km=4 x 105 M-1s-1)1-3. It is cleaved more slowly by the following cathepsins (kcat/Km: B (105)4, F (106)4, L (106)4,5, L2/V (104)5, S (105)2,4,5. Also cleaved by malaria parasite proteases berghepain6, vivapain-2 and -37, and falcipain-1, -2, and -36,8,9. Excitation: 365nm, Emission: 440nm. | | Uses | A sensitive fluorogenic substrate for cathepsin K | | References | Bossard et al. (1996), Proteolytic activity of human osteoclast cathepsin K. Expression, purification, activation, and substrate identification; J. Biol. Chem. 271 12517
Br?mme et al. (1996), Human cathepsin O2, a matrix protein-degrading cysteine protease expressed in osteoclasts. Functional expression of human cathepsin O2 in Spodoptera frugiperda and characterization of the enzyme; J. Biol. Chem. 271 2126
Linnevers et al. (1997), Expression of human cathepsin K in Pichia pastoris and preliminary crystallographic studies of an inhibitor complex; Protein Sci. 6 919
Wang et al. (1998), Human cathepsin F. Molecular cloning, functional expression, tissue localization, and enzymatic characterization; J. Biol. Chem., 273 32000
Br?mme et al. (1999), Human cathepsin V functional expression, tissue distribution, electrostatic surface potential, enzymatic characterization and chromosomal localization; Biochemistry, 38 2377
Singh et al. (2007), A chimeric cysteine protease of Plasmodium berghei engineered to resemble the Plasmodium falciparum protease falcipain-2; Protein Eng. Des. Sel. 20 171
Na et al. (2004), Identification and biochemical characterization of vivapains, cysteine proteases of the malaria parasite Plasmodium vivax; Biochem. J. 378(Pt2) 529
Goh et al. (2005), Cysteine protease falcipain 1 in Plasmodium falciparum is biochemically distinct from its isozymes; Parasitol. Res. 97 295
Pandey et al. (2004), Independent intramolecular mediators of folding, activity, and inhibition for the Plasmodium falciparum cysteine protease falcipain-2; J. Biol. Chem. 279 3484 |
| | Z-LEU-ARG-7-AMINO-4-METHYLCOUMARIN Preparation Products And Raw materials |
|