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| | N-Acetylneuraminic Acid Aldolase Basic information |
| Product Name: | N-Acetylneuraminic Acid Aldolase | | Synonyms: | EC 4.1.3.3;N-ACETYLNEURAMINIC ACID ALDOLASE;N-ACETYLNEURAMINIC ACID ALDORASE;N-ACETYLNEURAMINATE PYRUVATE LYASE;NEU5AC ALDOLASE;N-ACETYLNEURAMINIC ACID ALDOLASE, FROM MICROORGANISM;N-Acetylneuraminate Pyruvate Lyase, N-Acetylneuraminic Acid Lyase, NANA Aldolase, Sialic Aldolase;Sialic acid aldolase - Aqueous solution | | CAS: | 9027-60-5 | | MF: | | | MW: | 0 | | EINECS: | | | Product Categories: | Lyases;Specialty Enzymes | | Mol File: | Mol File | ![N-Acetylneuraminic Acid Aldolase Structure]() |
| | N-Acetylneuraminic Acid Aldolase Chemical Properties |
| | N-Acetylneuraminic Acid Aldolase Usage And Synthesis |
| Uses | This enzyme is useful for enzymatic determination of N-acetylneuraminic acid and sialic acid when
coupled with the related enzymes in clinical analysis.
For industrial use, this enzyme is useful for enzymatic synthesis of sialic acid. | | Uses | Sialic acid aldolase can be used to synthesize unnatural sugars of C(6) to C(10) for the design of antagonists and inhibitors of glycoenzymes. | | General Description | Sialic acid aldolases, or N-acetylneuraminate lyases, catalyze the reversible aldol cleavage of N-acetylneuraminic acid to form pyruvate and N-acetyl-D-mannosamine. In nature, N-acetylneuraminate lyase mainly occurs in pathogens. | | Purification Methods | Purify the aldolase by extraction with H2O, protamine precipitation, (NH4)2SO4 fractionation, Me2CO precipitation, acid treatment at pH 5.7 and precipitation at pH 4.5. The equilibrium constant for pyruvate + n-acetyl-D-mannosamine N-acetylneuraminidate at 37o is 0.64. The Km for N-acetylneuraminic acid is 3.9mM in phosphate at pH 7.2 and 37o. [Comb & Roseman Methods Enzymol 5 391 1962.] The enzyme from hog kidney (cortex) has been purified 1700-fold by extraction with H2O, protamine sulfate precipitation, (NH4)2SO4 fractionation, heating between 60-80o, a second (NH4)2SO4 fractionation and starch gel electrophoresis. The Km for N-acetylneuraminic acid is 1.5mM. [Brunetti et al. J Biol Chem 237 2447 1962.] |
| | N-Acetylneuraminic Acid Aldolase Preparation Products And Raw materials |
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