- SolventBlack3
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- $1.00 / 1KG
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2024-07-31
- CAS:4197-25-5
- Min. Order: 1KG
- Purity: 99%
- Supply Ability: 200000KG
- SOLVENT BLACK 3
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- $0.00 / 25kg
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2022-12-21
- CAS:4197-25-5
- Min. Order: 25kg
- Purity: 0.99
- Supply Ability: 20MT
- Solvent Black 3
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- $15.00 / 1KG
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2021-07-13
- CAS:4197-25-5
- Min. Order: 1KG
- Purity: 99%+ HPLC
- Supply Ability: Monthly supply of 1 ton
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| | Solvent Black 3 Basic information |
| Product Name: | Solvent Black 3 | | Synonyms: | LABOTEST-BB LT00451850;1H-Perimidine, 2,3-dihydro-2,2-dimethyl-6-((4-(phenylazo)-1-naphthyl)azo)-;Fast Black HB;Solvent Black 2;Sudan black b, high purity biological stain;SUDAN BLACK B (CERT);2,3-Dihydro-2,2-dimethyl-6-[[4-(phenylazo)naphthalen-1-yl]azo]-1H-perimidine;2,2-Dimethyl-6-{[4-(phenyldiazenyl)-1-naphthyl]-diazenyl}-2,3-dihydro-1H-perimidine | | CAS: | 4197-25-5 | | MF: | C29H24N6 | | MW: | 456.54 | | EINECS: | 224-087-1 | | Product Categories: | Azo;stain;Solvent Dyestuff;Organics | | Mol File: | 4197-25-5.mol |  |
| | Solvent Black 3 Chemical Properties |
| Melting point | 120-124 °C(lit.) | | Boiling point | 552.68°C (rough estimate) | | density | 1.4899 (rough estimate) | | refractive index | 1.4570 (estimate) | | storage temp. | Store at RT. | | solubility | Insoluble in water; soluble in ethanol, acetone, benzene, toluene, xylene, ethylene glycol | | form | Powder/Solid | | Colour Index | 26150 | | pka | 2.94±0.40(Predicted) | | color | Very dark brown to black | | Water Solubility | Soluble in oils, fats, warm petrolatum, paraffin, phenol, ethanol, acetone, benzene, toluene and hydrocarbon. Insoluble in water. | | λmax | 598 nm, 415 nm | | ε(extinction coefficient) | ≥20000 at 596-603nm in ethanol | | Merck | 13,8970 | | BRN | 723248 | | Stability: | Light Sensitive | | Biological Applications | Diagnosis of acute myeloid leukemia (AML); detecting neuron-specific nuclear protein NeuN; drug screening | | CAS DataBase Reference | 4197-25-5(CAS DataBase Reference) | | EPA Substance Registry System | C.I. Solvent Black 3 (4197-25-5) |
| Hazard Codes | F,Xi | | Risk Statements | 11-36/37/38 | | Safety Statements | 24/25-16-37/39-26 | | WGK Germany | 3 | | RTECS | SD4431500 | | TSCA | Yes | | HazardClass | IRRITANT | | HS Code | 32041900 | | Toxicity | LD50 ivn-mus: 63 mg/kg CSLNX* NX#04918 |
| | Solvent Black 3 Usage And Synthesis |
| Chemical Properties | It is a very dark brown to black fine crystalline powder. It is soluble in ethanol, toluene, acetone, and other solvents. In concentrated sulfuric acid, it is violet-black; when diluted, it is dark greenish-blue and produces a blue to black precipitate. The ethanol solution of the dye is bluish-black when concentrated hydrochloric acid is added and dark blue when concentrated sodium hydroxide solution is added. | | Uses | Sudan Black is a basic black dye that is used in staining triglycerides and lipids off some lipoproteins. Biological stain. Dyes and metabolites, Environmental Testing. | | Preparation | aniline diazotization, and Naphthalen-1-amine coupling, and its product to diazotization, and 2,2-Dimethyl-2,3-dihydro-1H-perimidine?coupling. | | Definition | ChEBI: Sudan black B is a member of the class of perimidines that is 2,2-dimethyl-2,3-dihydro-1H-perimidine carrying a [4-(phenyldiazenyl)naphthalen-1-yl]diazenyl substituent at position 6. A fat-soluble dye predominantly used for demonstrating triglycerides in frozen sections and for staining of protein bound lipids in paraffin sections. It has a role as a histological dye. It is a member of azobenzenes, a bis(azo) compound and a member of perimidines. | | Biological Activity | Sudan Black B has been used to stain phospholipids and intracellular lipids. Staining pattern is almost similar to myeloperoxidase reaction, with positive staining of granulocytic cells, eosinophils and weak monocytic staining.It doesn′t stain lymphocytes. Sudan Black B can be used to stain older blood or bone marrow smears, but the stain slightly fades over time. It is considered to be a most sensitive and specific reagent for lipid staining. Sudan Black B stains phospholipids gray and neutral fats blue-black. | | Safety Profile | A poison by intravenous route. When heated to decomposition it emits toxic vapors of NOx. | | Staining Procedures | 1 % Sudan black B is dissolved in 70% ethanol, the tissue is stained 1-1.5 h, subsequently washed in 70% ethanol (3 x 15 s), and mounted in 75% glycerol before microscopy. This staining method reveals positive results for lipids. | | Properties and Applications | Blue-black. Soluble in ethanol, acetone and toluene. In concentrated sulfuric acid for purple light black, dilution after deep green light blue, blue to black precipitation. Dye ethanol solution to join concentrated hydrochloric acid for blue black; Join concentrated sodium hydroxide solution for skipper.
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Standard
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Light Fastness
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Heat-resistant(℃)
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water
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Sodium Carbonate(5%)
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Hydrochloric acid(5%)
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Melting point
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Stable
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ISO
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General
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126
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100
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Insoluble
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No change
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No change
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| | Solvent Black 3 Preparation Products And Raw materials |
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