- Biotin-NHS ester
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- $0.00 / 25g
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2024-04-02
- CAS:35013-72-0
- Min. Order: 25g
- Purity: >95.00%
- Supply Ability: 25g
- BIOTIN-NHS
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- $15.00 / 1KG
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2021-07-13
- CAS:35013-72-0
- Min. Order: 1KG
- Purity: 99%+ HPLC
- Supply Ability: Monthly supply of 1 ton
- BIOTIN-NHS
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- $15.00 / 1KG
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2021-07-09
- CAS:35013-72-0
- Min. Order: 1KG
- Purity: 99%+ HPLC
- Supply Ability: Monthly supply of 1 ton
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| | BIOTIN-NHS Basic information |
| | BIOTIN-NHS Chemical Properties |
| Melting point | 212-214 °C | | density | 1.45±0.1 g/cm3(Predicted) | | storage temp. | -20°C | | solubility | DMF: ≤50 mg/mL Stable for at least one month in dry DMF | | form | powder | | pka | 13.89±0.40(Predicted) | | color | White to off-white | | BRN | 4720781 | | Stability: | Stable. Incompatible with strong oxidizing agents. | | CAS DataBase Reference | 35013-72-0(CAS DataBase Reference) |
| | BIOTIN-NHS Usage And Synthesis |
| Description | N-Hydroxysuccinimide (NHS) activates carboxylic acid groups (on biotin) to facilitate coupling reactions. Biotin-NHS is a compound used to attach biotin to primary amines under alkaline conditions (pH~8-9). For example, lysines on the surface of proteins, including antibodies, are ideal targets for biotinylation with this compound. | | Chemical Properties | White Solid | | Uses | Biotin-NHS is used in the biotinylation of proteins and peptides. Couples with primary amine in the pH range 6.5-8.5. | | Uses | amine reactive biotinylation reagent | | Uses | Amino reactive biotin reagent for preparing biotinylated surfaces or polypeptides. | | Purification Methods | Recrystallise the ester from refluxing isoPrOH and dry it in a vacuum over P2O5 + KOH. [Jasiewicz et al. Exp Cell Biol 100 213 1976.] |
| | BIOTIN-NHS Preparation Products And Raw materials |
| Raw materials | 1H-Imidazole, 1-[5-[(3aS,4S,6aR)-hexahydro-2-oxo-1H-thieno[3,4-d]imidazol-4-yl]-1-oxopentyl]--->TSTU-->D-Biotin | | Preparation Products | biotinylamidoethylacetamide-->LC-LC(+)-Biotin-->N-BIOTINYL-3,6,9-TRIOXAUNDECANE-1,11-DIAMINE-->D-BIOTINOL-->(+)-BIOTIN-EPSILON-AMINOCAPROIC ACID-->FMOC-LYS(BIOTIN)-OH-->4-((Biotinoyl)amino)Butyric acid-->N-[6-[[5-[(3aS,4S,6aR)-Hexahydro-2-oxo-1H-thieno[3,4-d]imidazol-4-yl]-1 -oxopentyl]amino]hexyl]-carbamic Acid 1,1-Dimethylethyl Ester |
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