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Sigma-Aldrich
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021-61415566 800-8193336 |
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| Products Intro: |
Product Name:Tth DNA Polymerase
Purity:Tth DNA Polymerase Package:1EA Remarks:11480022001
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| | TTH DNA POLYMERASE Basic information |
| Product Name: | TTH DNA POLYMERASE | | Synonyms: | TTH DNA POLYMERASE;THERMOSTABLE REVERSE TRANSCRIPTASE;RTTH DNA POLYMERASE;Q-THERMORT(TM) | | CAS: | | | MF: | | | MW: | 0 | | EINECS: | | | Product Categories: | | | Mol File: | Mol File | ![TTH DNA POLYMERASE Structure]() |
| | TTH DNA POLYMERASE Chemical Properties |
| | TTH DNA POLYMERASE Usage And Synthesis |
| Uses | Thermus thermophilus (Tth) DNA Polymerase might be used:
- to amplify DNA fragments by polymerase chain reaction (PCR) due to its resistance towards prolonged incubations at high temperatures (95 °C)
- to label DNA fragments with either radiolabeled nucleotides, digoxigenin, or biotin, since this enzyme accepts modified deoxyribonucleotides as substrates
- to efficiently transcribe RNA targets into cDNA due to its intrinsic Mn-dependent reverse transcriptase (RT) activity
- for real time PCR
| | General Description | Tth DNA Polymerase is isolated from the thermophilic eubacterium Thermus thermophilus HB8, and is purified to be free of nonspecific DNases and RNases. The enzyme is a highly processive 5′-3′ DNA polymerase, and lacks 3′-5′ exonuclease activity. The enzyme exhibits its highest activity at a pH of approximately 9 (adjusted at +25°C), and temperatures around +75°C. It is resistant to prolonged incubations at high temperatures (+95°C). | | Biochem/physiol Actions | Thermus thermophilus (Tth) DNA Polymerase is a thermostable DNA polymerase with intrinsic reverse transcriptase (RT) activity. The enzyme is a highly processive 5′-3′ DNA polymerase and lacks 3′-5′ exonuclease activity (proof reading). It was found to possess a very efficient intrinsic reverse transcriptase (RT) activity in the presence of manganese ions – much higher than that demonstrated for Escherichia coli DNA polymerase I and Taq DNA Polymerase. The RT activity is not associated with RNase H activity. The elevated temperatures of Tth DNA Polymerase (optimum +55°C to +70°C, maximum +95°C) activity overcomes the problems posed by RNA secondary structure. Resulting cDNA can be amplified by PCR using the same enzyme in the presence of Mg2+-ions. The ability of Tth DNA Polymerase to perform both reverse transcription and DNA amplification at elevated temperatures allows this enzyme to be used for quantitative RT-PCR, cloning, and gene expression analysis of cellular and viral RNA. Tth DNA Polymerase is used for RT-PCR amplification of RNA up to 1kb. |
| | TTH DNA POLYMERASE Preparation Products And Raw materials |
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