Orexin B, human TFA
| 中文名称 | Orexin B, human TFA |
|---|---|
| 中文同义词 | 内源性激动剂H-ARG-SER-GLY-PRO-PRO-GLY-LEU-GLN-GLY-ARG-LEU-GLN-ARG-LEU-LEU-GLN-ALA-SER-GLY-ASN-HIS-ALA-ALA-GLY-ILE-LEU-THR-MET-NH2 |
| 英文名称 | Orexin B, human TFA |
| 英文同义词 | Orexin B, human TFA |
| CAS号 | |
| 分子式 | C???H???N??O??S.C?HF?O? |
| 分子量 | 0 |
| EINECS号 | |
| 相关类别 | 目录多肽 |
| Mol文件 | Mol File |
| 结构式 |
Orexin B, human TFA 性质
| 形态 | 固体 |
|---|---|
| 颜色 | 白色至米白色 |
Ki: 420 nM (OX1), 36 nM (OX2).
Orexin B is derived by proteolytic amino acid precursor, prepro-orexin, which is encoded by a gene localized to chromosome 17q21 in humans. In radioligand binding studies, Orexin B has a higher affinity for the OX2 receptor.
Human Orexin B has two amino acid substitutions when compared with the rodent sequence. OX2 receptor is indeed a high-affinity receptor for human orexin B, with an IC
50
of 36 nM in the binding assay and an EC
50
of 60 nM in the [Ca
2+
]i transient assay. Human Orexin B has significantly lower affinity for the human OX1: the calculated IC
50
in the competitive binding assay and the EC
50
in the [Ca
2+
]i transient assay are 420 nM and 2500 nM for human orexin-B, respectively.
Human Orexin B significantly augments food intake; at the 2 hr time point, 5- and 12-fold stimulation of food consumption is observed by 3 nM and 30 nM orexin-B, respectively.
| Animal Model: | Male Wistar rats (180-200 g). |
| Dosage: | 3 nM and 30 nM. |
| Administration: | Administered in a 5 mL bolus through a catheter placed in the left lateral ventricle in early light phase. |
| Result: | Significantly augmented food intake. |