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Cytochrome C

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CAS:9007-43-6
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CAS:9007-43-6

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  • Cytochrome C
  • US $0.00 / g
  • 2021-09-29
  • CAS:9007-43-6
  • Min. Order: 1KG
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  • Cytochrome C
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  • Cytochrome C
  • US $1.00 / KG
  • 2019-07-06
  • CAS:9007-43-6
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Cytochrome C Basic information
Product Name:Cytochrome C
Synonyms:Cytochromes C from equine heart;HUMAN CYTOCHROME C;CYT-C;CYTOCHROME C;CYTOCHROME C, ACID MODIFIED;CYTOCHROME C, BOVINE;CYTOCHROME C (BOVINE HEART);CYTOCHROME C PEPTIDE MAP CONTROL
CAS:9007-43-6
MF:C42H52FeN8O6S2
MW:884.88728
EINECS:232-700-9
Product Categories:Cytochrome c;Cytochromes;Electron Transport and Cellular Respiration;Cytochrome cProteomics;Mass Spectrometry;Peptide and protein standards for mass spectrometry analysis;Antibiotic Explorer;Cytochrome c Control ProteinsMass Spectrometry;Peptide Mapping Controls;Enzymatic and Chemical Protein Cleavage;API
Mol File:9007-43-6.mol
Cytochrome C Structure
Cytochrome C Chemical Properties
Melting point 300 °C
storage temp. -20°C
solubility H2O: 10 mg/mL, clear, dark red-brown
form powder
Water Solubility 100 g/L (20 ºC)
Merck 13,2820
EPA Substance Registry SystemCytochrome c (9007-43-6)
Safety Information
Hazard Codes Xi,B,Xn
Risk Statements 36/37/38-20/21/22
Safety Statements 26-36-37/39-24/25
WGK Germany 3
RTECS HA5365000
1-8-10
TSCA Yes
HS Code 35040000
MSDS Information
ProviderLanguage
Cytochrome C English
SigmaAldrich English
ACROS English
Cytochrome C Usage And Synthesis
Chemical PropertiesBrown-red crystalline powder
Definitioncytochrome: Any of a group ofproteins, each with an iron-containinghaem group, that form part ofthe electron transport chain in mitochondriaand chloroplasts. Electronsare transferred by reversiblechanges in the iron atom betweenthe reduced Fe(II) and oxidized Fe(III)states.
Industrial usesCytochrome c is involved in the mitochondrial electron-transfer chain and accepts an electron from cytochrome c1 and transfers it to cytochrome c oxidase. This electron is subsequently used in the reduction of oxygen, where four electrons are needed. This means that actually four cytochrome c transfer an electron to cytochrome c oxidase, where one molecule of O2 is converted to two molecules of water.
Safety ProfileExperimental reproductive effects. Mutation data reported. When heated to decomposition it emits toxic fumes of NOx. See also OXALATES.
Purification MethodsCytochrome c1 is purified by chromatography on CM-cellulose (CM-52 Whatman) [Brautigan et al. Methods Enzymol 53D 131 1978]. It has a high PI (isoelectric point) and has been purified further by adsorption onto an acidic cation exchanger, e.g. Amberlite IRC-50 (polycarboxylic) or in ground form Amberlite XE-40 (100-200 mesh) or Decalso-F (aluminium silicate), where the non-cytochrome protein is not adsorbed and is readily removed. The cytochrome is eluted using a solution containing 0.25g ions/L of a univalent cation at pH 4.7 adsorbed onto the NHsalt of Amberlite IRC-50 at pH 7, washed with H2O and then with 0.12M NH4OAc to remove non-cytochrome protein. When the cytochrome begins to appear in the eluate, then the NH4OAc concentration is increased to 0.25 M. The fractions with ca Fe = 0.465—0.467 are collected, dialysed against H2O and adsorbed onto a small IRC-50 column and eluted with 0.5M NH3, then dialysed and lyophilised. (A second fraction II can be eluted from the first resin with 0.5M NH3 but is discarded). [Keilin & Hartree Biochemical Preparations 1 1 1952, Margoliash Biochemical Preparations 8 33 1957.] Cytochrome c has been recrystallised as follows: The above eluate (ca 100mL) is dialysed against H2O (10 vols) at 4o for 24hours (no more), then passed through an XE-40 column (2 x 1 cm above) which is equilibrated with 0.1M NH4OAc pH 7.0. The column is washed with 0.1% (NH4)2SO4 pH 8.0, and the dark red resin in the upper part of the column is collected and in 0.1% (NH4)2SO4 pH 8.0 is transferred to another column (7mm diameter) and the cytochrome c is eluted with 5% (NH4)2SO4 pH 8.0. More than 98% of the red colour is collected in a volume of ca 4mL in a weighed centrifuge tube. Add a drop of octanol and 0.43g of (NH4)2SO4/g of solution. When the salt has dissolved, ascorbic acid (5mg) is added as well as a few drops of 30% NH3, and it is kept at 10o for 10minutes (turns lighter colour due to reduction). Then add finely powdered (NH4)2SO4 in small portions (stir with a glass rod) until the solution becomes turbid. Stopper the tube tightly, and set aside at 15-25o for 2days while the cytochrome c separates as fine needles or rosettes. Further (NH4)2SO4 (20mg) are added per mL of suspension and kept in the cold for a few days to complete the crystallisation. The crystals are collected by centrifugation (5000xg), suspended in saturated (NH4)2SO4 (pH 8.0 at 10o), then centrifuged again. For recrystallisation the crystals are dissolved in the least volume of H2O, one drop of ammonia and 1 mg of ascorbic acid are added and the above process is repeated. The yield of twice recrystallised cytochrome c from 2Kg of muscle is ca 200 mg, but this varies with the source and freshness of the muscle used. The crystals are stored as a solid after dialysis against 0.08M NaCl or 0.1M sodium buffer and lyophilising, or as a suspension in saturated (NH4)2SO4 at 0o. [Hagihara et al. Biochemical Preparations 6 1 1958.] Purity of cytochrome c: This is checked by the ratio of the absorbance at 500nm (reduced form) to 280nm (oxidised form), i.e. should be between 1.1 and 1.28, although values of up to 1.4 have been obtained for pure preparations. For the preparation of the reduced form see Margoliash Biochemical Preparations 5 33 1957 and Yonetani Biochemical Preparations 11 19 1966. Cytochrome from Rhodospirillum rubrum ( 551 0.967) is purified by chromatography on a column of 270/ CM-Whatman cellulose [Paleus & Tuppy Acta Chem Scand 13 641 1959].
Cytochrome C Preparation Products And Raw materials
Tag:Cytochrome C(9007-43-6) Related Product Information
CYTOCHROME C OXIDASE ASSAY KIT CYTOCHROME P450 2C19 ISOZYME HUMAN CYTOCHROME P450 2C9 (HUMAN),CYTOCHROME P450 2C9 ISOZYMES HUMAN,CYTOCHROME P450 2C9 PROTEIN (HUMAN) CYTOCHROME P450 2C8 ISOZYME MICROSOMES HUMAN TRIS(2,2,6,6-TETRAMETHYL-3,5-HEPTANEDIONATO)EUROPIUM(III) Aluminum acetylacetonate N-BUTYLISOCYANIDE Tris(2,4-pentanedionato)chroMiuM(III) Ethyl isocyanoacetate Ferric acetylacetonate Tosylmethyl isocyanide Cupric acetylacetonate TRIS(2,2,6,6-TETRAMETHYL-3,5-HEPTANEDIONATO)DYSPROSIUM(III) COBALT ETHYLENE DIAMINE CHLORIDE DICHLORO(ETHYLENEDIAMINE)PLATINUM(II) TERT-BUTYL ISOCYANIDE 2,4-PENTANEDIONE, SILVER DERIVATIVE COBALT(II) ACETYLACETONATE