| Company Name: |
Sigma-Aldrich
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| Tel: |
021-61415566 800-8193336 |
| Email: |
orderCN@merckgroup.com |
| Products Intro: |
Product Name:MG-132, Ready Made Solution
Purity:>=90% (HPLC) Package:200UL Remarks:M7449-200UL
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| Company Name: |
Riedel-de Haen AG
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| Tel: |
800 558-9160 |
| Email: |
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| Products Intro: |
Purity:90% (HPLC)
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| | MG-132, Ready Made Solution Basic information |
| | MG-132, Ready Made Solution Chemical Properties |
| Fp | 87 °C | | storage temp. | -20°C | | form | DMSO solution |
| | MG-132, Ready Made Solution Usage And Synthesis |
| Uses | MG-132, Ready Made Solution has been used to inhibit the proteasome in various systems, including:
- hTERT-RPE1 and HeLa cells: Osorio-Zambrano, W.F. et al., PLoS One, 10(12), e0144434 (2015)
- FaDu human hypopharynx cancer cells: Kim, S.-Y. et al., Autophagy, 14(3), 385-403 (2018)
- HEK293 cells: Chen, Q. et al., Cell Reports, 22(11), 2860-2872 (2018).
- Neonate rat cardiomyocytes: Turkieh, A. et al., Cell Death Dis., 10(8), 608 (2019)
- Adult neural stem cells: Morrow, C.S. et al., Cell Stem Cell, 26(4), 558-568.e9 (2020).
| | General Description | MG132 is a potent proteasome inhibitor that also inhibits calpains and lysosomal cathepsins. One of the first proteasome inhibitors to be developed, MG-132 is widely used in proteasome inhibition studies because of its high potency, high selectivity, and rapid reversibility. MG132 mechanistically acts as a proteasome inhibitor by hemiacetal formation with hydroxyl groups of active site threnonine residues. | | Biochem/physiol Actions | MG132, also known as carbobenzoxy-Leu-Leu-leucinal, is a peptide aldehyde which plays a vital role in the inhibition of proteolytic activity of the 26S proteasome complex. MG132 retards the growth of HeLa cells by promoting cell cycle arrest and apoptosis. Treatment of HepG2 and HeLa cells with MG-132 triggers increased expression of MCPIP1 mRNA, which in turn increases MCPIP1 protein concentration. MCPIP1 is implicated in negative regulation of macrophage activation and differentiation of several cell types, including pre-adipocytes, neuroprogenitor cells and osteoclast precursors. MG-132 stimulates dopamine reduction and nigral dopaminergic degeneration in both cell culture and animal models of Parkinson′s disease. |
| | MG-132, Ready Made Solution Preparation Products And Raw materials |
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