| Company Name: |
Sigma-Aldrich
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| Tel: |
021-61415566 800-8193336 |
| Email: |
orderCN@merckgroup.com |
| Products Intro: |
Product Name:Insulin-transferrin-sodium selenite media supplement
Purity:gamma-irradiated, lyophilized powder, BioXtra, suitable for Package:1VL Remarks:I1884-1VL
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| Company Name: |
Riedel-de Haen AG
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| Tel: |
800 558-9160 |
| Email: |
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| Products Intro: |
Remarks:γ-irradiated, lyophilized powder, BioXtra, suitable for cell culture
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| Company Name: |
SIGMA-RBI
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| Tel: |
800 736 3690 (Orders) |
| Email: |
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| Products Intro: |
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| | ITS Supplement Basic information |
| Product Name: | ITS Supplement | | Synonyms: | Insulin-transferrin-sodium selenite media supplement;ITS Supplement | | CAS: | | | MF: | | | MW: | 0 | | EINECS: | | | Product Categories: | | | Mol File: | Mol File | ![ITS Supplement Structure]() |
| | ITS Supplement Chemical Properties |
| | ITS Supplement Usage And Synthesis |
| Uses | Insulin-transferrin-sodium selenite media supplement has been used as:
- medium 199 (M199) media supplement for cardiac myocytes culture
- Roswell Park Memorial Institute (RPMI) 1640 medium component for pluripotent stem cell (iPSC) culture
- Dulbecco′s Modified Eagle Medium (DMEM) supplement in ID8 ovarian carcinoma cells
| | General Description | Serum-free and reduced serum formulations often require purified forms of the factors normally supplied by serum that are suitable for in vitro growth and differentiation studies. These important factors include insulin, transferrin, selenium, pyruvate, and ethanolamine.
INSULIN is a polypeptide hormone that promotes the uptake of glucose and amino acids and may owe observed mitogenic effect to this property.
TRANSFERRIN is an iron-transport protein. Iron is an essential trace element, but can be toxic in the free form. To nourish cells in culture, it is supplied bound to transferrin in serum.
SELENIUM is an essential trace element normally provided by Serum.
Nutritional studies indicate that the supplement components described are utilized by most mammalian cells. They enhance cell proliferation and decrease the serum requirement of many cell types. When the provided supplement is used with 2 to 4 percent serum, proliferation is reported to be similar to medium supplemented with 10 percent serum. |
| | ITS Supplement Preparation Products And Raw materials |
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