灵芝酸D
中文名称 | 灵芝酸D |
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中文同义词 | 灵芝酸D;灵芝酸D对照品,;灵芝酸D标准品 |
英文名称 | Ganoderic acid D |
英文同义词 | (7beta)-7-Hydroxy-3,11,15,23-tetraoxolanost-8-en-26-oic acid;Lanost-8-en-26-oic acid, 7-hydroxy-3,11,15,23-tetraoxo-, (7β)-;cyclophilin,colon,CypD,Ganoderic acid D,human,Apoptosis,inhibit,oxygenated,Sirtuin,tetracyclic,cancer,carcinoma,HeLa,triterpenoid,Inhibitor,deacetylated,cervical |
CAS号 | 108340-60-9 |
分子式 | C30H42O7 |
分子量 | 514.65 |
EINECS号 | |
植物来源 | 灵芝 |
相关类别 | 三萜;植物提取物;对照品,标准品;中药对照品;标准品;对照品;对照品-中药对照品;标准品 -中药标准品;标准品-对照品;标准品,对照品;chemical reagent;pharmaceutical intermediate;phytochemical;reference standards from Chinese medicinal herbs (TCM).;standardized herbal extract |
Mol文件 | 108340-60-9.mol |
结构式 |
灵芝酸D 性质
沸点 | 688.3±55.0 °C(Predicted) |
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密度 | 1.22±0.1 g/cm3(Predicted) |
储存条件 | Store at -20°C, protect from light |
溶解度 | 溶于甲醇; |
形态 | 粉末 |
酸度系数(pKa) | 4.77±0.23(Predicted) |
颜色 | 白色 |
水溶解性 | insoluble in water |
SIRT3
|
Ganoderic acid D can inhibit the growth of numerous cancer cell lines and it inhibits HeLa human cervical carcinoma cells with an IC
50
of 17.3 mM.
Ganoderic acid D (1-50 μM; 24-72 hours) reduces the cell survival rate in a dose- and time-dependent manner.
Ganoderic acid D (10, 50 μM; 24, 48 hours) induces G2/M phase arrest.
Ganoderic acid D (10, 50 μM; 24, 48 hours) induces a morphological change typical of apoptosis in HeLa cells.
Ganoderic acid D (10 μM; 48 hours) up-regulates 14-3-3E and PRDX3.
Cell Viability Assay
Cell Line: | HeLa human cervical carcinoma cell line (CCL-2) |
Concentration: | 1, 5, 10, 20, 50 μM |
Incubation Time: | 24, 48, 72 hours |
Result: | Reduced the cell survival rate in a dose- and time-dependent manner and had an IC 50 value of 17.3 μM for 48 hours treatment. |
Cell Cycle Analysis
Cell Line: | HeLa human cervical carcinoma cell line (CCL-2) |
Concentration: | 10, 50 μM |
Incubation Time: | 24, 48 hours |
Result: |
Induced G2/M phase arrest.
Displayed a cell cycle profile with an elevated G2/M cell population after 24-h treatment with 10 μM. |
Apoptosis Analysis
Cell Line: | HeLa human cervical carcinoma cell line (CCL-2) |
Concentration: | 10, 50 μM |
Incubation Time: | 48 hours |
Result: | Induced a morphological change typical of apoptosis in HeLa cells. |
Western Blot Analysis
Cell Line: | HeLa human cervical carcinoma cell line (CCL-2) |
Concentration: | 10 μM |
Incubation Time: | 48 hours |
Result: | Up-regulated 14-3-3E and PRDX3. |
药理药效:抗肿瘤,保肝,心血管方面作用