- 6-FAM SE
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- $43.00 / 10mg
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2025-09-11
- CAS:92557-81-8
- Min. Order:
- Purity: 98.23%
- Supply Ability: 10g
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| | 6-CARBOXYFLUORESCEIN N-SUCCINIMIDYL ESTER Basic information |
| | 6-CARBOXYFLUORESCEIN N-SUCCINIMIDYL ESTER Chemical Properties |
| Melting point | >168°C (dec.) | | Boiling point | 788.1±70.0 °C(Predicted) | | density | 1.74±0.1 g/cm3(Predicted) | | storage temp. | −20°C | | solubility | Soluble in N,N-dimethylformamide,dimethyl sulfoxide | | form | Solid | | pka | 9.31 ± 0.20, most acidic, temperature:25 °C | | color | Orange solid | | Appearance | Yellow solid | | ex/em | 492/517 nm | | ε(extinction coefficient) | 74,000 L⋅mol−1⋅cm−1 | | Φ(quantum yield) | 0.93 | | λmax | 496 nm (Buffer pH9) | | Stability: | Moisture Sensitive | | Biological Applications | Analyzing
aminoglycosides;
analyzing/determining gabapentin;
detecting/analyzing baclofen in human plasma at
micromolar level;
determining phosphorus-containing
amino acid herbicides;
multiplex genetic analysis;
visualizing/detecting proteins; for synthesizing
vancomycin analogs,
vasopressin analogs;
as a substrate for measuring kinase activity, phosphatase
activity. | | InChI | InChI=1S/C25H15NO9/c27-13-2-5-16-19(10-13)33-20-11-14(28)3-6-17(20)25(16)18-9-12(1-4-15(18)24(32)34-25)23(31)35-26-21(29)7-8-22(26)30/h1-6,9-11,27-28H,7-8H2 | | InChIKey | VDABVNMGKGUPEY-UHFFFAOYSA-N | | SMILES | C12(C3=C(C=C(O)C=C3)OC3=C1C=CC(O)=C3)C1=C(C=CC(C(ON3C(=O)CCC3=O)=O)=C1)C(=O)O2 | | CAS DataBase Reference | 92557-81-8(CAS DataBase Reference) |
| | 6-CARBOXYFLUORESCEIN N-SUCCINIMIDYL ESTER Usage And Synthesis |
| Description | Fluorescein (FAM) is a bright fluorophore that has found an extremely wide application in life science research. The fluorophore is hydrophilic and its derivatives possess good solubility in aqueous buffers.
Fluorescein is compatible with a wide variety of fluorescent instrumentation - most instruments are equipped with FAM channel filters.
This product contains the pure 6-isomer of fluorescein. | | Chemical Properties | Yellow to orange powder | | Uses | Intracellular pH probe. Fluorescent label for amines and alcohols. | | Uses | (6-FAM-SE) 6-Carboxyfluorescein-N-hydroxysuccinimide Ester is a commonly used fluorescent reagent for the labelling of peptides, proteins and nucleotides. | | Definition | ChEBI: 6-carboxyfluorescein succinimidyl ester is an N-hydroxysuccinimide ester derived from 6-carboxyfluorescein. A fluorescent cell staining dye, it is cell permeable and covalently couples, via its succinimidyl group, to intracellular molecules, notably to intracellular lysine residues. It has a role as a fluorochrome. It is functionally related to a fluorescein (lactone form). | | Abs/Em Maxima | 492/517 nm | | Fluoresene quantum yield | 0.93 | | Extinction Coefficient | 74000 | | CF260 | 0.22 | | CF280 | 0.17 |
| | 6-CARBOXYFLUORESCEIN N-SUCCINIMIDYL ESTER Preparation Products And Raw materials |
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