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PCR DIG Probe Synthesis Kit

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PCR DIG Probe Synthesis Kit Suppliers list
Company Name: Sigma-Aldrich  
Tel: 021-61415566 800-8193336
Email: orderCN@merckgroup.com
Products Intro: Product Name:PCR DIG Probe Synthesis Kit
Purity:PCR DIG Probe Synthesis Kit Package:1EA Remarks:11636090910
PCR DIG Probe Synthesis Kit Basic information
Product Name:PCR DIG Probe Synthesis Kit
Synonyms:PCR DIG Probe Synthesis Kit
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MW:0
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PCR DIG Probe Synthesis Kit Structure
PCR DIG Probe Synthesis Kit Chemical Properties
storage temp. -20°C
Safety Information
MSDS Information
PCR DIG Probe Synthesis Kit Usage And Synthesis
UsesDigoxigenin (DIG)-labeled DNA probes produced from the PCR DIG Probe Synthesis Kit is suitable for low-(single)-copy gene detection of rare mRNA in Southern and northern blots. DIG-labeled DNA probes have also been used for labeling of DNA during in situ hybridization.
The concentration of the supplied dUTP-nucleotide mix can be adjusted according to probe length. Labeling effectiveness can quickly be determined on an agarose gel.
Stripping and reprobing of membranes is possible multiple times following the protocol in the package insert.
One PCR labeling reaction (50 μl) will typically yield enough probe for 20 ml hybridization solution. The kit can be used for approximately 25 reactions (50 μl).
General DescriptionThe PCR DIG Probe Synthesis Kit contains an alkali-labile digoxigenin (DIG)-11deoxyuridinetriphosphate (dUTP) formulation. This kit is for convenient and efficient generation of DIG-labeled DNA probes that are highly sensitive in polymerase chain reaction (PCR). The probes are labeled with DIG-11dUTP (alkali-labile), by the method of PCR. The ratio of DIG-dUTP:dTTP is 1:2.
The PCR DIG Probe Synthesis Kit contains the Expand High Fidelity DNA polymerase mix. This robust enzyme mix with proofreading activity will polymerize probes 40 bp to 5 kb long using 10 pg plasmid DNA and 10 ng genomic DNA as template. The DIG-labeled probes are stable for over one year.
PCR DIG Probe Synthesis Kit Preparation Products And Raw materials
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