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Alkaline Phosphatase

Alkaline Phosphatase Suppliers list
Company Name: Henan DaKen Chemical CO.,LTD.
Tel: +86-371-55531817
Email: info@dakenchem.com
Products Intro: Product Name:EC 3.1.3.1
CAS:9001-78-9
Purity:99% Package:100g,500g,1kg,5kg,10kg
Company Name: Henan Tianfu Chemical Co.,Ltd.
Tel: 0371-55170693
Email: info@tianfuchem.com
Products Intro: CAS:9001-78-9
Purity:99% Package:500G;1KG;5KG;25KG
Company Name: career henan chemical co
Tel: +86-371-86658258
Email: sales@coreychem.com
Products Intro: Product Name:Alkaline Phosphatase
CAS:9001-78-9
Purity:99% Package:1KG;1USD
Company Name: Haihang Industry Co.,Ltd
Tel: 86-531-88032799
Email: export@haihangchem.com
Products Intro: Product Name:Alkaline Phosphatase
CAS:9001-78-9
Company Name: Zhonglan Industry Co., Ltd.
Tel: (86) 531-82956570
Email: sales@zhonglanindustry.com
Products Intro: Product Name:Alkaline Phosphatase (AP), origin bovine intestine
CAS:9001-78-9

Lastest Price from Alkaline Phosphatase manufacturers

  • Alkaline Phosphatase
  • US $1.00 / KG
  • 2019-07-06
  • CAS:9001-78-9
  • Min. Order: 1KG
  • Purity: 99%
  • Supply Ability: 25kg
Alkaline Phosphatase Basic information
Product Name:Alkaline Phosphatase
Synonyms:alkaline;phosphatasealkalinefrome.coli;ALKALINE PHOSPHATASE, SHRIMP;ALKALINE PHOSPHATASE, BOVINE;ALKALINE PHOSPHATASE CALF INTESTINAL;EC 3.1.3.1;EC: 3.1.3.1;CALF INTESTINAL ALKALINE PHOSPHATASE
CAS:9001-78-9
MF:C21H36N8O6
MW:496.56054
EINECS:232-631-4
Product Categories:enzyme;HPLC Fittings;SSI Fittings;Stainless Steel Fittings
Mol File:9001-78-9.mol
Alkaline Phosphatase Structure
Alkaline Phosphatase Chemical Properties
density 1.12 g/mL at 20 °C
storage temp. 2-8°C
form suspension
Specific Gravity1
color white
Water Solubility It is soluble in water.
Stability:Stability Incompatible with strong oxidizing agents
EPA Substance Registry SystemAlkaline phosphatase (9001-78-9)
Safety Information
Hazard Codes B,Xi
Risk Statements 36/37/38
Safety Statements 26-36-24/25-22-23
RIDADR UN 0029 1.1B
RIDADR UN 0030 1.1B
WGK Germany 3
3-10
TSCA Yes
HazardClass 1.1B
HazardClass 1.1B
HS Code 35079090
MSDS Information
ProviderLanguage
SigmaAldrich English
Alkaline Phosphatase Usage And Synthesis
Chemical Propertiessolid
UsesHydrolyzes 5′-terminal monophosphate (dephosphorylation) from DNA and RNA. Prevents fragments from self annealing. 5′-nucleic acid targeting for probes.
UsesAlkaline Phosphatase is commonly used to remove the 5′-terminal phosphate from nucleic acids.
Purification MethodsThe E.coli supernatant in sucrose (20%, 33mM) in Tris-HCl pH 8.0 is purified through a DEAE-cellulose column and recrystallised. To the column eluates in 0.125M NaCl is added MgCl2 (to 0.01M) and brought to 50% saturation in (NH4)2SO4 by adding the solid (0.20g/mL). The mixture is centrifuged to remove bubbles and is adjusted to pH 8.0 (with 2N NaOH). Saturated (NH4)2SO4 at pH 8.0 is added dropwise until the solution becomes faintly turbid (~61% saturation). It is set aside at ~25o for 1hour (turbidity will increase). The mixture is placed in an ice bath for several minutes when turbidity disappears and a clear solution is obtained. It is then placed in a large ice bath at 0o (~5L) and allowed to warm slowly to room temperature in a dark room whereby crystals are formed appearing as a silky sheen. The crystals are collected by centrifugation at 25o if necessary. The crystalline solutions are stable at ~25o for many months. They can be stored at 0o, but are unstable when frozen. Cysteine at 10-3M and thioglycolic acid at 10-4M are inhibitory. This is reversed on addition of Zn2+ ions. Many organic phosphates are good substrates for this phosphatase. [Molamy & Horecker Methods Enzymol 9 639 1966, Torriani et al. Methods Enzymol 12b 212 1968, Engstrom Biochim Biophys Acta 92 71 1964.] Alkaline phosphatase from rat osteosarcoma has been purified by Me2CO precipitation and chromatography on DEAE-cellulose, Sephacryl S-200, and hydroxylapatite. [Nair et al. Arch Biochem Biophys 254 18 1987.] Phosphoproteins (various). These are purified by adsorbing onto an iminodiacetic acid substituted agarose column to which are bound ferric ions. This chelate complex acts as a selective immobilised metal affinity adsorbent for phosphoproteins. [Muszyfiska et al. Biochemistry 25 6850 1986.]
Alkaline Phosphatase Preparation Products And Raw materials
Raw materials1-Butanol
Tag:Alkaline Phosphatase(9001-78-9) Related Product Information
ALKALINEPHOSPHATASE(ALP)(EC3.1.3.1) Cupric acetylacetonate Ferric acetylacetonate PHENYLSELENOL COBALT(II) ACETYLACETONATE 2,4-PENTANEDIONE, SILVER DERIVATIVE TERT-BUTYL ISOCYANIDE Tosylmethyl isocyanide N-BUTYLISOCYANIDE SALCOMINE TRIS(2,2,6,6-TETRAMETHYL-3,5-HEPTANEDIONATO)DYSPROSIUM(III) COBALT ETHYLENE DIAMINE CHLORIDE Aluminum acetylacetonate DICHLORO(ETHYLENEDIAMINE)PLATINUM(II) 1,1,3,3-TETRAMETHYLBUTYL ISOCYANIDE METHYL ISOCYANOACETATE TRIS(2,2,6,6-TETRAMETHYL-3,5-HEPTANEDIONATO)EUROPIUM(III) BENZYL ISOCYANIDE