| Company Name: |
Suzhou Zhixin Biotechnology Co., Ltd. Gold
|
| Tel: |
0512-65118909 15162312715 |
| Email: |
sales@szzxbio.com |
| Products Intro: |
Product Name:5-Ph-IAA
CAS:168649-23-8
Purity:98%+ Package:1g;10g;100g
|
|
| | 1H-Indole-3-acetic acid, 5-phenyl- Basic information |
| Product Name: | 1H-Indole-3-acetic acid, 5-phenyl- | | Synonyms: | 1H-Indole-3-acetic acid, 5-phenyl-;5-Ph-IAA;5-(2-phenyl)-indole-3-acetic acid;inhibit,antitumor,OsTIR1,Inhibitor,auxin,5 Ph IAA,5PhIAA,AID2;2-(5-Phenyl-1H-indol-3-yl)acetic acid | | CAS: | 168649-23-8 | | MF: | C16H13NO2 | | MW: | 251.28 | | EINECS: | | | Product Categories: | | | Mol File: | 168649-23-8.mol |  |
| | 1H-Indole-3-acetic acid, 5-phenyl- Chemical Properties |
| Boiling point | 521.3±35.0 °C(Predicted) | | density | 1.291±0.06 g/cm3(Predicted) | | storage temp. | Store at -20°C | | solubility | DMSO: 125 mg/mL (497.45 mM) | | form | A solid | | pka | 4.47±0.30(Predicted) |
| | 1H-Indole-3-acetic acid, 5-phenyl- Usage And Synthesis |
| Biological Activity | 5-Ph-IAA is a derivative of IAA. 5-Ph-IAA is a ligand that together with the OsTIR1 (F74G) mutant establishes the auxin-inducible degron 2 system (AID2). AID2 induces rapid and efficient depletion of mAID fusion proteins to study protein function in living cells. AID2 can suppress tumors. | | in vitro | HCT116 cells constitutively expressing OsTIR1(F74G) are exposed to 5-Ph-IAA (1 μM; 6 hours), leading to DHC1-mAC degradation, which means the AID2 system better than the original AID system in generating conditional mutant cell lines. Western Blot Analysis | Cell Line: | HCT116 cells | | Concentration: | 1 μM | | Incubation Time: | 6 hours | | Result: | Depleted DHC1-mAC efficiently in HCT116 cells constitutively expressing OsTIR1(F74G). | | | in vivo | 5-Ph-IAA (0-10 mg/kg; intraperitoneally injection; every day; 7 days), used for the AID2 system , is sufficient to deplete mAID-BRD4 and TOP2A-mAC, leading to tumor suppression. | Animal Model: | Balb/c-nu female mice (7 weeks old; 16–20 g ) bearing mAID-BRD4 and TOP2A-mAC xenograft tumours cells | | Dosage: | 0 mg/kg, 1 mg/kg , 3 mg/kg and 10 mg/kg | | Administration: | Intraperitoneally injection; every day; 7 days | | Result: | Displayed significant tumor suppression of mAID-BRD4 xenografts and TOP2A-mAC xenografts . | | | storage | Store at -20°C |
| | 1H-Indole-3-acetic acid, 5-phenyl- Preparation Products And Raw materials |
|