2',7'-Dichlorofluorescein diacetate NEW
| Price | $30 | $39 | $51 |
| Package | 25mg | 50mg | 100mg |
| Min. Order: | |
| Supply Ability: | 10g |
| Update Time: | 2025-12-05 |
Product Details
| Product Name: 2',7'-Dichlorofluorescein diacetate | CAS No.: 2044-85-1 |
| Purity: 99.60% | Supply Ability: 10g |
| Release date: 2025/12/05 |
Product Introduction
Bioactivity
| Name | 2',7'-Dichlorofluorescein diacetate |
| Description | 2',7'-Dichlorofluorescein diacetate (Diacetyldichlorofluorescein), a cell-permeable fluorogenic probe, is useful for the detection of nitric oxide (NO) and reactive oxygen species (ROS) and for the determination of the degree of overall oxidative stress. |
| In vitro | I. Detection of Reactive Oxygen Species (ROS) 1. Material preparation 1) DCFH-DA probe solution: Prepare DCFH-DA stock solution (1–10 mM), dissolve it in anhydrous DMSO, and store it away from light. 2) Cell sample: Living cells or tissue samples to be tested. 3) PBS buffer: Used to wash cells, pH 7.4. 2. Experimental steps 1) Cell treatment: Collect the cells to be tested, wash them with PBS 2-3 times to remove interferences in the culture medium. 2) Staining: Dilute DCFH-DA to 0.1–20 µM, add it to the cell culture medium, incubate at 37°C for 30 minutes, and protect from light. 3) Washing: After incubation, wash the cells with PBS buffer to remove DCFH-DA that has not entered the cells. 4) Detect fluorescence: Use a fluorescence microscope or flow cytometer to detect the fluorescence signal. Excitation wavelength: 488 nm Emission wavelength: 525 nm II. Detection of Nitric Oxide (NO) DCFH-DA detects the presence of NO through an indirect mechanism, depending on the secondary products generated by NO (such as peroxynitrite). The experimental steps are similar to ROS detection and are suitable for indirect assessment of changes in NO content. III. Assessment of total oxidative stress 1) Compare the fluorescence signal with the standard curve to quantitatively determine the level of ROS or NO. 2) Use blank groups (cells not treated with DCFH-DA) and positive controls (such as H2O2 or NO donor treatment groups) to verify the reliability of the experimental results. |
| Storage | keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature. |
| Solubility Information | DMSO : 5.79 mg/mL (11.93 mM), Sonication is recommended. |
| Keywords | ROS | reactive oxygen | oxidative stress | Leuco-dcf Diacetate | Leuco-dcf | Inhibitor | inhibit | DCFH2-DA | cell-permeable | 2′,7′-Dichlorofluorescein diacetate | 2',7'-Dichlorofluorescein Diacetate | 2',7'Dichlorofluorescein diacetate | 2',7'-Dichlorofluorescein | 2',7' Dichlorofluorescein diacetate |
| Inhibitors Related | Ethoxyquin | Kaolin | Allopurinol | Cysteamine hydrochloride | Acetylcysteine | Imeglimin hydrochloride | L-Ascorbic acid | Glucosamine | Inosine | L-Cystine | L-Ascorbic acid sodium salt | Sodium 2-oxopropanoate |
| Related Compound Libraries | Human Metabolite Library |
Company Profile Introduction
Target Molecule Corp. (TargetMol) is a global high-tech enterprise, headquartered in Boston, MA, specializing in chemical and biological research product and service to meet the research needs of global customers.
TargetMol has evolved into one of the biggest global compound library and small molecule suppliers and a customer based on 40+ countries. TargetMol offers over 80 types of compound libraries and a wide range of high-quality research chemicals including inhibitors, activator, natural compounds, peptides, inhibitory antibodies, and novel life-science kits, for laboratory and scientific use. Besides, virtual screening service is also available for customers who would like to conduct the computer-aided drug discovery.
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United States- Since: 2011-01-07
- Address: 36 Washington Street, Wellesley Hill, MA
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