ACRIDINE ORANGE NEW

Price	$5	$0.2
Package	1KG	1000KG

Min. Order:	1KG
Supply Ability:	g-kg-tons, free sample is available
Update Time:	2024-03-26

Product Details

Product Name: ACRIDINE ORANGE	CAS No.: 65-61-2
Min. Order: 1KG	Purity: 98%
Supply Ability: g-kg-tons, free sample is available	Release date: 2024/03/26
Lead time: In stock, ready for shipment	Packaging: bag/bottle/drum/IBC
Delivery: By express, by air, by sea	Origin: Manufacturer, advantage product
COA, MSDS: Available, contact us for details	Name: Tina

1. Materials information

Names

Name	acridine orange
Synonym	More Synonyms

Description	Acridine orange is a cell-permeable fluorescent dye that binds to nucleic acids, resulting in an altered spectral emission.
Related Catalog	Signaling Pathways >> Others >> Others Dye Reagents Research Areas >> Others
In Vitro	Acridine orange has been employed extensively as a cytochemical stain and has shown to stain differentially DNA and RNA, and double-restranded nucleic acids in situ. Acridine orange can either intercalate into double helical nucleic acids (green fluorescence at 530 nm), or bind electrostatically to phosphate groups of single-stranded molecules (red fluorescence at 640 nm). This unique characteristic makes acridine orange useful for cell-cycle studies[1]. Acridine orange staining of unfixed cells may be used as a simple, fast means of obtaining information on cell ploidy levels and cell cycle status from DNA measurements (green fluorescence), and cell transcriptional activity from RNA staining (red fluorescence), in human and murine cells lines, peripheral blood and bone marrow specimens from patients with leukemia and mitogenically (phytohemagglutinin) or antigenically (mixed lymphocyte culture) stimulated human peripheral blood cultures[2].
Cell Assay	Two-step, pH 3.0: Aliquots (0.2 mL, containing approximately 2-5x105 cells) are withdrawn from cultures and are added to 0.5 mL of a solution containing: 0.1% (v/v) Triton X-100, 0.2 M sucrose, 10-4 M EDTA and 2x10-2 M citrate-phosphate buffer, at pH 3.0. Triton X-100 is included in the various procedures at the indicated pH to increase cell permeability yet maintain cellular integrity. The chelating agent EDTA is used to facilitate RNA denaturation. The cells are stained one minute later by addition of 1 mL of a solution containing 0.002% (20 μg/mL) AO, 0.1 M NaCl and 10-2 M citrate-phosphate buffer, pH 3.8. Cations are included in the staining mixture to ensure staining specificity. The final AO concentration is approximately 4x10-5 M[2].
References	[1]. McMaster GK, et al. Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarosegels by using glyoxal and acridine orange. Proc Natl Acad Sci U S A. 1977 Nov;74(11):4835-8. [2]. Traganos F, et al. Simultaneous staining of ribonucleic and deoxyribonucleic acids in unfixed cells using acridine orange in a flow cytofluorometric system. J Histochem Cytochem. 1977 Jan;25(1):46-56.

Density	1.001 g/mL at 20ºC
Boiling Point	468.6ºC at 760 mmHg
Melting Point	284-287ºC(lit.)
Molecular Formula	C₁₇H₂₀ClN₃
Molecular Weight	301.81400
Flash Point	237.2ºC
Exact Mass	301.13500
PSA	19.37000
LogP	4.32200
Index of Refraction	n20/D 1.338
Water Solubility	Soluble in water, ethanol, dimethyl sulfoxide

Acridine Orange hydrochloride MSDS(Chinese)

CHEMICAL IDENTIFICATION

RTECS NUMBER :
AR7601000

CHEMICAL NAME :
Acridine, 3,6-bis(dimethylamino)-, monohydrochloride

CAS REGISTRY NUMBER :
65-61-2

LAST UPDATED :
199701

DATA ITEMS CITED :
20

MOLECULAR FORMULA :
C17-H19-N3.Cl-H

MOLECULAR WEIGHT :
301.85

WISWESSER LINE NOTATION :
T C666 BNJ EN1&1 MN1&1 &GH

HEALTH HAZARD DATA

ACUTE TOXICITY DATA

TYPE OF TEST :
Sex chromosome loss and nondisjunction

TYPE OF TEST :
Micronucleus test

MUTATION DATA

TYPE OF TEST :
DNA adduct

TEST SYSTEM :
Mammal - species unspecified Lymphocyte

DOSE/DURATION :
10 pph

REFERENCE :
BIPMAA Biopolymers. (John Wiley & Sons, Inc., 605 Third Ave., New York, NY 10158) V.1- 1963- Volume(issue)/page/year: 11,2537,1972 *** NIOSH STANDARDS DEVELOPMENT AND SURVEILLANCE DATA *** NIOSH OCCUPATIONAL EXPOSURE SURVEY DATA : NOES - National Occupational Exposure Survey (1983) NOES Hazard Code - X3803 No. of Facilities: 29 (estimated) No. of Industries: 1 No. of Occupations: 3 No. of Employees: 625 (estimated) No. of Female Employees: 403 (estimated) NOES - National Occupational Exposure Survey (1983) NOES Hazard Code - X8883 No. of Facilities: 10 (estimated) No. of Industries: 1 No. of Occupations: 1 No. of Employees: 114 (estimated) No. of Female Employees: 31 (estimated)

Personal Protective Equipment	Eyeshields;Gloves;half-mask respirator (US);multi-purpose combination respirator cartridge (US)
Safety Phrases	S22-S24/25
RIDADR	NONH for all modes of transport
WGK Germany	3
RTECS	AR7601000
HS Code	32041300

2. Packaging of materials

For powders: normal is 25kgs/Drum or bag, or larger/smaller package as request.
For liquids: normal 25kgs/drum, 180-300kgs/bucket, or IBC, determined by the nature of the product.

Or smaller package 1kg/bottle, 10kgs/bottle as request.

Article illustration

3. Shipping & Delivery

By Express

Provide door to door service

Suitable for goods under 50kg

Delivery: 3-7 days

Cost: low cost

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By Air

Provide airport to airport service

Suitable for goods over 50kg

Delivery: 3-14 days

Cost: high cost

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By Sea

Provide seaport to seaport service

Suitable for goods over 100kg

Delivery: 2-45 days

Cost: low cost

Article illustration

4. Contact information

For more details, pls contact us freely.

Email address: Tina@fdachem.com

Mob: 86 15225627621

WhatsApp/Skype/Wechat/LINE: 86 15225627621

Company Profile Introduction

Henan Fengda Chemical Co., Ltd. is located in the High-tech Development Zone of Henan Province. Specializing in the production and sales of various fine chemical products required for industrial production, including chemical raw materials, organic raw materials, petrochemicals, chemical reagents, solvents, catalysts, and additives, etc.

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