HADA Hydrochloride NEW
| Price | $97 | $247 | $397 |
| Package | 1mg | 5mg | 10mg |
| Min. Order: | |
| Supply Ability: | 10g |
| Update Time: | 2025-10-20 |
Product Details
| Product Name: HADA Hydrochloride | CAS No.: 2253733-10-5 |
| Purity: 98.02% | Supply Ability: 10g |
| Release date: 2025/10/20 |
Product Introduction
Bioactivity
| Name | HADA Hydrochloride |
| Description | HADA Hydrochloride (HCC-Amino-D-alanine hydrochloride) is a blue (λem~450 nm) fluorescent D-amino acid (FDAA). FDAAs are efficiently incorporated into the peptidoglycans (PGs) of diverse bacterial species at the sites of PG biosynthesis. HADA Hydrochloride can be used in specific and covalent probing of bacterial growth with minimal perturbation. |
| Cell Research | HADA labeled PG site experiment of E. coli cells I. Solution preparation Mother solution preparation: Prepare 50 mM (100×) stock solution with DMSO; (it is recommended to store at -20 ℃ or -80 ℃ in the dark after aliquoting) II. Operation steps 1. Inoculate cells from -80°C bacterial cryopreservation solution into sterile liquid culture medium and culture cells using optimal species-specific conditions until exponential growth is achieved. 2. Add 10 μL of 50 mM HADA DMSO stock solution to the culture tube. 3. Dilute the culture to OD600 = 0.05 in fresh LB; 4. Add 990 μL of the culture to the culture tube containing FDA to obtain a final LB solution of [HADA] = 500 μM and [DMSO] = 1%, and incubate the culture at 37°C with shaking; 5. When the culture reaches OD600 = 0.4 – 0.6, pellet the cells (1 min, maximum speed in a microcentrifuge, room temperature); 6. Resuspend the cells in 1 mL 70% (vol/vol) ice-cold EtOH and fix the cells on ice for 10 – 15 min. |
| In vitro | FDAA labeling can be completed in as little as 30 seconds for rapidly growing species like Escherichia coli. Despite HADA Hydrochloride being dimmer and less photostable compared to FDL or TDL, it consistently and effectively labels the PG of a wide range of bacterial species, usually without requiring significant optimization[1]. |
| Storage | keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature. |
| Solubility Information | DMSO : 112.5 mg/mL (342.2 mM), Sonication is recommended. |
| Keywords | HCC-Amino-D-alanine Hydrochloride | HADA Hydrochloride |
| Inhibitors Related | Neomycin sulfate | Aceglutamide | Cysteamine hydrochloride | Adipic dihydrazide | Sulfamethoxazole sodium | Terbinafine hydrochloride | Hydroxychloroquine | Metronidazole | Copper(Ⅱ) Sulfate | Paeonol | Alginic acid | BES |
Company Profile Introduction
Target Molecule Corp. (TargetMol) is a global high-tech enterprise, headquartered in Boston, MA, specializing in chemical and biological research product and service to meet the research needs of global customers.
TargetMol has evolved into one of the biggest global compound library and small molecule suppliers and a customer based on 40+ countries. TargetMol offers over 80 types of compound libraries and a wide range of high-quality research chemicals including inhibitors, activator, natural compounds, peptides, inhibitory antibodies, and novel life-science kits, for laboratory and scientific use. Besides, virtual screening service is also available for customers who would like to conduct the computer-aided drug discovery.
You may like
Recommended supplier
| Product name | Price | Suppliers | Update time | |
|---|---|---|---|---|
| $2.00/100kg |
VIP1Y
|
ZHENGZHOU JIUYI TIME NEW MATERIALS CO,.LTD
|
2025-06-19 | |
| $0.00/1kg |
VIP4Y
|
Dingwang Technology (Wuhan) Co., Ltd.
|
2022-10-28 | |
| $0.00/1kg |
VIP1Y
|
Chongqing Soarwin Technology Co., Ltd
|
2025-04-09 |
United States- Since: 2011-01-07
- Address: 36 Washington Street, Wellesley Hill, MA
INQUIRY