N-(2-hydroxyethyl)-Naphthalimide NEW
| Price | $47 | $67 |
| Package | 50mg | 100mg |
| Min. Order: | |
| Supply Ability: | 10g |
| Update Time: | 2025-09-18 |
Product Details
| Product Name: N-(2-hydroxyethyl)-Naphthalimide | CAS No.: 5450-40-8 |
| Purity: 100.00% | Supply Ability: 10g |
| Release date: 2025/09/18 |
Product Introduction
Bioactivity
| Name | N-(2-hydroxyethyl)-Naphthalimide |
| Description | N-(2-hydroxyethyl)-Naphthalimide is a fluorescent probe for nucleic acid detection whose fluorescence can be burst. |
| Animal Research | I. Application in nucleic acid detection 1. Material preparation (1) HEN probe: dissolved in an appropriate organic solvent (such as DMSO or ethanol), the recommended concentration is 1-10 µM. (2) Sample: extracted DNA/RNA, or single-stranded nucleic acid for in vitro synthesis (such as specific oligonucleotides). (3) Buffer: buffer suitable for nucleic acid reaction (such as Tris-HCl, pH 7.5). 2. Experimental steps (1) Establishment of fluorescence detection system: Prepare the reaction system, including HEN probe and target nucleic acid, and ensure uniform mixing. The binding of probe to nucleic acid can quench the fluorescence signal by forming a complex or changing the surrounding environment. (2) Fluorescence signal monitoring: Record the change of fluorescence intensity on a fluorescence spectrometer, the excitation wavelength is usually 350-400 nm, and the emission wavelength is 450-500 nm. Evaluate the nucleic acid concentration or reaction kinetics by the change of fluorescence signal. (3) Nucleic acid comparison experiment: Compare the target nucleic acid sequence with the non-specific nucleic acid sequence to verify the selectivity and specificity of the probe. c. Data analysis Use the change in fluorescence signal intensity (or quenching efficiency) to draw a fluorescence titration curve, and calculate the concentration of the target molecule by nonlinear fitting. 2. Fluorescence imaging Experimental steps: (1) Load the HEN probe into the cell culture medium and incubate with living cells. (2) Use a fluorescence microscope to observe the distribution of the probe in the cell nucleus or cytoplasm and record the fluorescence intensity. (3) For specific nucleic acid targets, control experiments can be performed by adding RNA/DNA enzymes to verify the binding specificity of the probe. |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature. |
| Solubility Information | DMSO : 30 mg/mL (124.36 mM), Sonication is recommended. Ethanol : 1 mg/mL (4.15 mM), Sonication is recommended. DMF : 30 mg/mL (124.36 mM), Sonication is recommended. DMSO:PBS (pH 7.2) (1:1) : 0.5 mg/mL (2.07 mM), Sonication is recommended. |
| Keywords | N-(2-hydroxyethyl)-Naphthalimide | N(2hydroxyethyl)Naphthalimide | N (2 hydroxyethyl) Naphthalimide |
Company Profile Introduction
Target Molecule Corp. (TargetMol) is a global high-tech enterprise, headquartered in Boston, MA, specializing in chemical and biological research product and service to meet the research needs of global customers.
TargetMol has evolved into one of the biggest global compound library and small molecule suppliers and a customer based on 40+ countries. TargetMol offers over 80 types of compound libraries and a wide range of high-quality research chemicals including inhibitors, activator, natural compounds, peptides, inhibitory antibodies, and novel life-science kits, for laboratory and scientific use. Besides, virtual screening service is also available for customers who would like to conduct the computer-aided drug discovery.
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