N-Nitroso Indacaterol NEW

N-Nitroso Indacaterol

WhatsAPP: +86 17386083646
E-mail: anna@molcoo.com

Product Information

• Product Number: I018023

• English Name: N-Nitroso Indacaterol

• English Alias: (R)-N-(5,6-diethyl-2,3-dihydro-1H-inden-2-yl)-N-(2-hydroxy-2-(8-hydroxy-2-oxo-1,2-dihydroquinolin-5-yl)ethyl)nitrous amide

• CAS Number: None

• Molecular Formula: C₂₄H₂₇N₃O₄

• Molecular Weight: 421.49

Advantages

• Well-defined with distinct chirality: Contains (R)-chiral indane core, 5,6-diethyl substituents, quinolinone ring (8-hydroxy, 2-oxo), hydroxyethyl side chain, and nitrous amide (-N-NO). Unlike indacaterol (long-acting β₂ agonist), its nitrous amide polarity and polycyclic hydrophobicity create significant physicochemical differences, enabling precise differentiation via HPLC/chiral chromatography as a specific marker;

• High stability and traceability: Rigid indane-quinolinone structure and stability of hydroxyl/nitrous amide ensure stability under dark, low-temperature conditions. As a derivative from amine nitrosation during storage/synthesis, it directly reflects raw material purity and nitrite exposure, improving impurity tracing accuracy;

• High detection sensitivity: Polycyclic conjugation shows strong UV absorption (250-300nm), combined with m/z 422 [M+H]⁺ enabling ppb-level analysis via LC-MS, compatible with long-acting β₂ agonist nitroso impurity systems.

Applications

• Pharmaceutical quality control: Used as an impurity reference standard to quantify N-Nitroso Indacaterol in APIs and formulations, ensuring compliance with genotoxic impurity limits in pharmaceutical standards;

• Storage optimization: Monitoring impurity levels under varying conditions (pH, humidity) to assess indacaterol stability and prevent excessive nitrosation;

• Synthesis assessment: Evaluating purity of amine intermediates in indacaterol synthesis to reduce nitrosation risk at the source.

Background Description

Research Status

• Analytical method validation: Developing UPLC-MS/MS methods with C18 columns for separation, achieving 0.01 ppb detection limits;

• Nitrosation mechanism: Studying impurity formation kinetics under varying nitrite concentration and pH to clarify secondary amine-to-nitrous amide conversion pathways;

• Control strategies: Exploring nitrosation inhibitors (e.g., ascorbic acid) to keep impurity levels below safety limits (<0.001%);

• Toxicity evaluation: Conducting in vitro genotoxicity tests (e.g., Ames test) to assess potential hazards and support limit setting.

We can also customize related analogues and modified peptides including HPLC, MS, 1H-NMR, MS, HPLC, IR, UV, COA, MSDS.
This product is intended for laboratory use only!

WhatsAPP: +86 17386083646
E-mail: anna@molcoo.com