N-Nitroso rac-Proline NEW

N-Nitroso rac-Proline

Product Information

• Product Code：N031288

• English Name：N-Nitroso rac-Proline

• English Alias：1-nitrosopyrrolidine-2-carboxylic acid

• CAS No.：2571-28-0

• Molecular Formula：C₅H₈N₂O₃

• Molecular Weight：144.13

Advantages

• High-Purity Reference Standard：Confirmed by HPLC (≥99.0%), NMR (1H, 13C), HRMS, and elemental analysis, suitable for precise analysis of N-nitroso impurities.

• Stability Assurance：Stable for 36 months at -20℃ under light-protected, sealed storage; degradation rate <0.2% in acetonitrile-water solution within 6 months.

Applications

• Quality Control Testing：Used for UPLC-MS/MS detection of N-nitroso impurities in proline-containing drugs, food additives, and cosmetics, controlling content to meet international regulatory standards (e.g., ≤10 μg/kg in food).

• Biological Sample Analysis：Detects N-Nitroso rac-Proline in human urine, blood, and other biological samples to assess in-vivo exposure levels of nitrosocompounds, with an LOD of 0.01 ng/mL.

• Process Optimization Research：Monitors N-Nitroso rac-Proline formed by nitrosation side reactions during proline derivative synthesis or fermentation, reducing generation by >70% by adjusting reaction pH (6-7) and avoiding nitrite exposure.

Background Description

Research Status

• Detection Technology：UPLC-MS/MS with C18 column (1.7μm) and 0.1% formic acid-acetonitrile gradient elution achieves separation within 2.5 minutes, with LOD of 0.003 ng/mL for trace nitrosamine analysis.

• Formation Mechanism：Formed by reaction of proline with sodium nitrite under acidic conditions (pH<4) or indirectly via microbial metabolism of nitrites. Adding antioxidants (e.g., vitamin C) and optimizing fermentation processes inhibit its formation.

• Safety Evaluation：Animal studies show carcinogenicity, with a permitted daily intake (TTC value) determined as 1.5 μg/day. Current population cohort studies analyze the correlation between its exposure levels and cancer incidence.

We can also customize related analogues and modified peptides including HPLC, MS, 1H-NMR, MS, HPLC, IR, UV, COA, MSDS.

This product is intended for laboratory use only!

WhatsAPP: +86 17320513646
E-mail: anna@molcoo.com

NEW IN STOCK!

The Molcoo Laboratory added drug impurity reference standards, including Baricitinib, Piperazine, Benzylpenicillin, Tranilast and multiple N-Nitroso drug impurities! Now available for immediate delivery!