Recombinant Carboxypeptidase B NEW

product overview

Carboxypeptidase B is a metal carboxypeptidase that specifically hydrolyzes the amino terminus of basic amino acids (lysine, arginine, histidine) at the C-terminus of proteins or peptides. This product is expressed and produced by recombinant Escherichia coli, with an amino acid sequence identical to that of rat pancreatic carboxypeptidase B. It has the same enzymatic properties as animal derived carboxypeptidase B and can be used as a substitute for animal derived carboxypeptidase B in various biotechnology processes. The optimal pH for CPB is 7.5-9.0. Both acidic and alkaline conditions can cause a decrease in activity. When the pH is close to 5.0, the activity approaches zero, and when the pH is greater than 12.0, the activity is completely lost. Arginine, lysine, and metal ion chelating agents such as EDTA can inhibit their activity.

Product advantages

1. Adequate production capacity: The 5-ton production line produces multiple batches, with batch production reaching kg level, and the quality is verified to be stable through multiple batches of production;

2. GMP level: The production process meets GMP requirements;

3. Complete quality documents: It can ensure that there is no animal origin, no external virus contamination, etc., and the quality documents are complete.

4. Complete quality inspection system: equipped with specific enzyme activity and enzyme residue detection kits, providing on-site inspection support and impurity risk control strategies.

5. Customization of specifications: Specifications can be customized according to customer needs.

storage condition

Storage conditions: 2~8 ℃ (sealed);

Retest period: 24 months.

Product application scope

1. Used for the production and preparation of recombinant insulin and its analogues, GLP-1 drugs;

2. Used for the production and preparation of recombinant peptides;

3. Protein C-terminal amino acid sequencing.

Suggested reaction system

Enzyme quantity: Target protein (mass ratio)=1:50-1:1000, reaction pH 7.5-9.0, enzyme digestion effect detected by SDS-PAGE or HPLC.

precautions

1. It can be dissolved in sterile water or 20 mM Tris HCl buffer at pH 7.6 to 1-10 mg/ml, and subjected to repeated freeze-thaw cycles 5 times without any loss of activity.

2. The recommended reaction system is based on the experimental conditions obtained by our company, and can be adjusted according to the actual situation during use.

Company Profile

    Our company is a high-tech enterprise specializing in chemical raw materials such as electronic materials, optoelectronic materials, semiconductor materials, UV monomers, silane catalysts, cosmetic raw materials, etc. The company integrates research and development, production, customized synthesis, and sales, and is committed to providing customers with high-quality chemical product solutions.

Our Company

Our Advantage

Quality control

Based on industry norms, customer requirements, and internal quality objectives, we establish clear quality standards. By monitoring the production process in real-time, we ensure that every step conforms to the predetermined quality standards.

Excellent quality is one of the cornerstones of our success. We welcome ordering samples for quality testing.

Technological innovation

Our technological innovation is reflected in the development of new materials that are at the leading edge of the industry. These materials not only possess higher performance and lower costs but also enable sustainable use within an environmentally friendly production process.

Competitiveness

Based on rich experience in material development and production, we have established a more scientific cost control system. By ensuring product quality, and through refined management and continuous process optimization, we offer more competitive prices.

Word of mouth

Adhering to the principle of customer first, customer first, and honest service, we have established long-term business relationships with customers and are deeply welcomed and trusted by them.

Rich experience

Our products have been exported to many countries, including Germany, Spain, the United Kingdom, the United States, Australia, the Middle East, Asia, and more. We have received highly positive feedback from our clients and have established long-term friendly cooperative relationships with them.

Safe and fast delivery

We will arrange shipment of spot products as soon as we receive payment.

Customized products are determined based on the synthesis time of different products.

Excellent pre-sales and after-sales service

Pre sales:

We are committed to providing the most favorable quotes and detailed information about our products and company.

After-sale service:

We assist buyers in customs clearance by providing necessary documents and information.

If there is any dispute over product quality, we are committed to providing the best solution.

Our packaging and shipping

• Packaging: 

1kg/bag or bottle, 25kg/drum, 50kg/drum, 200L/drum, 1T/bag or IBC drum, standard export packaging or packaging required by customers.

• Shipping

• Delivery:

Within 7 days after receiving your payment

Contact information

For more details, pls contact us freely.

Name: Elin

Email: jiuyitime@fdachem.com

 Sincerely hope to establish friendly business relationships with new and old customers, and jointly create a beautiful future for the fine chemical industry!