寡核苷酸是短的、单链或双链的DNA或RNA分子。其合成方法发展历程：从Michelson和Todd早期的H-磷酸盐和磷酸三酯法（H-Phosphonate & Phosphotriester）；到1950年代Khorana的磷酸二酯法（Phosphodiester）；以及1960-1970年代的磷酸三酯法（Phosphotriester）和亚磷酸三酯法（Phosphite triester）的发展。而直至1980年代，Marvin Caruthers的亚磷酰胺法（Phosphoramidite），加上自动化固相技术，成为了目前主流的寡核苷酸合成技术方法。在聚合酶链反应(PCR)、核酸测序、基因检测、核酸药物研发等领域有广泛应用。
亚磷酰胺寡聚体的合成在3'-5'方向进行(与生物合成中DNA复制的5'-3'方向相反)。每个合成周期添加一个核苷酸。亚磷酰胺DNA合成循环如图所示。
亚磷酰胺DNA合成循环
保护核苷
产品号 |
中文名称 |
英文名称 |
级别 |
Cas |
规格 |
N2-异丁酰基-2′-脱氧鸟苷
N2-Isobutyryl-2′-deoxyguanosine
98%
68892-42-2
100mg,250mg,1g,5g
5'-O-(4,4'-二甲氧基三苯基)-N2-异丁酰基-2'-脱氧鸟苷
N2-Isobutyryl-5′-O-(4,4′-dimethoxytrityl)-2′-deoxyguanosine
99.00%
68892-41-1
1g,5g,10g,25g,100g
N6-二苯甲酰基腺苷-2',3'-二苯甲酸酯
N6-Dibenzoyladenosine 2',3'-Dibenzoate
>98.0%(HPLC)(T)
58463-04-0
100mg,1g
N4-苯甲酰基-5′-O-(4,4′-二甲氧基三苯基)-2′-脱氧胞苷
N4-Benzoyl-5′-O-(4,4′-dimethoxytrityl)-2′-deoxycytidine
99%
67219-55-0
1g,5g
N4-苯甲酰胞苷
N4-Benzoylcytidine
98%
13089-48-0
1g,5g,25g
N4-苯甲酰基-2'-脱氧胞苷
N4-Benzoyl-2′-deoxycytidine
98.00%
4836-13-9
250mg,1g,5g
N6-苯甲酰基-2'-脱氧腺苷水合物
N6-Benzoyl-2′-deoxyadenosine hydrate
98%
206752-42-3,4546-72-9
250mg,1g,5g
N6-苯甲酰基-5′-O-(4,4′-二甲氧基三苯基)-2′-脱氧腺苷
N6-Benzoyl-5′-O-(4,4′-dimethoxytrityl)-2′-deoxyadenosine
99%
64325-78-6
100mg,1g,5g
5′-O-(4,4′-二甲氧基三苯甲基)胸苷
5′-O-(4,4′-Dimethoxytrityl)thymidine
98.00%
40615-39-2
1g,5g,25g
N6-苯甲酰基腺苷
N-Benzoyladenosine
97%
4546-55-8
1g,5g,25g
核糖，2'-脱氧核糖
产品号 |
中文名称 |
英文名称 |
级别 |
Cas |
规格 |
L-核糖
L-(+)-Ribose
98%
24259-59-4
1g,5g,10g,25g,100g
四乙酰核糖
1,2,3,5-Tetra-O-acetyl-β-D-ribofuranose
98%
13035-61-5
5g,25g,100g,500g
D-(-)-核糖
D-(-)-Ribose
≥99%（HPLC)
50-69-1
5g,25g,100g,250g,500g
D-(-)-核糖
D-(-)-Ribose
98%
50-69-1
100g,500g,2.5Kg
1-乙酰基-2,3,5-三苯甲酰氧基-1-beta-D-呋喃核糖
β-D-Ribofuranose 1-acetate 2,3,5-tribenzoate
98%
6974-32-9
1g,5g,25g,100g
甲基-β-D-呋喃核糖苷
Methyl β-D-Ribofuranoside
>97.0%(HPLC)
7473-45-2
1g,5g,25g,100g
3,5-二-O-(对甲苯酰)-2-脱氧-Α-D-呋喃核糖氯
3,5-Di-O-(p-toluyl)-2-deoxy-D-ribofuranosyl chloride
90%
4330-21-6
250mg
2-脱氧-D-核糖
2-Deoxy-D-Ribose
98%
533-67-5
1g,5g,25g,100g
2-脱氧-2,2-二氟-D-赤-戊糖酸γ-内酯3,5-二苯甲酸酯
2-Deoxy-2,2-difluoro-D-erythro-pentonic Acid γ-Lactone 3,5-Dibenzoate
98%
122111-01-7
1g,5g,25g,100g
2-脱氧-2-氟-1,3,5-三-O-苯甲酰基-α-D-阿拉伯呋喃糖
2-Deoxy-2-fluoro-1,3,5-tri-O-benzoyl-α-D- arabinofuranose
-
97614-43-2
1g,5g,25g
硫转移试剂
产品号 |
中文名称 |
英文名称 |
级别 |
Cas |
规格 |
氢化黄原素
Xanthane Hydride
>95.0%(HPLC)
6846-35-1
5g,25g,100g,500g
3H-1,2-苯并二硫醇-3-酮1,1-二氧化物
3H-1,2-Benzodithiol-3-one 1,1-Dioxide
>98.0%(HPLC)
66304-01-6
250mg,1g,5g
双(苯乙酰)二硫化物
Bis(phenylacetyl) Disulfide
>98.0%(HPLC)
15088-78-5
5g,25g
磷酸化试剂，磷硫化试剂
产品号 |
中文名称 |
英文名称 |
级别 |
Cas |
规格 |
二氯磷酸甲酯
Methyl Phosphorodichloridate
>95.0%(T)
677-24-7
5g,25g,100g
2-氰乙基N,N,N′,N′-四异丙基亚磷酰二胺
2-Cyanoethyl N,N,N′,N′-tetraisopropylphosphordiamidite
97%
102691-36-1
1g,5g,25g,100g
焦磷酸四苄酯
Tetrabenzyl Pyrophosphate
>98.0%(HPLC)
990-91-0
250mg,1g,5g,25g
二氯化磷酸苯酯
Phenyl dichlorophosphate
98%
770-12-7
25g,100g,500g
二(四丁铵)焦磷酸二氢盐
Bis(tetrabutylammonium) Dihydrogen Pyrophosphate
>96.0%(T)
857447-79-1
50mg,200mg
3H-1,2-苯并二硫醇-3-酮1,1-二氧化物
3H-1,2-Benzodithiol-3-one 1,1-Dioxide
>98.0%(HPLC)
66304-01-6
250mg,1g,5g
2-氯-1,3,2-二氧磷杂环戊烷 2-氧化物
2-Chloro-1,3,2-dioxaphospholane-2-oxide
≥95.0%
6609-64-9
5g,25g,100g
氯硫代磷酸二乙酯
Diethyl Chlorothiophosphate
>97.0%(GC)
2524-04-1
5g,25g,100g
2-氯苯基二氯膦酸酯[磷酸化剂]
2-Chlorophenyl Phosphorodichloridate [Phosphorylating Agent]
>97.0%(T)
15074-54-1
1g,5g,25g,100g
2-氯-4H-1,3,2-苯并二氧磷-4-酮
2-Chloro-4H-1,3,2-benzodioxaphosphorin-4-one
>95.0%
5381-99-7
1g,5g,25g
氯代磷酸二苯酯
Diphenyl phosphoryl chloride
97%
2524-64-3
25g,100g,500g
二氯亚磷酸甲酯[亚磷酸化剂]
Methyl Dichlorophosphite [Phosphitylating Agent]
>95.0%(T)
3279-26-3
5g,10g,25g,100g
双(苯乙酰)二硫化物
Bis(phenylacetyl) Disulfide
>98.0%(HPLC)
15088-78-5
5g,25g
4-氯苯基二氯磷酸酯
4-Chlorophenyl Phosphorodichloridate
>97.0%(T)
772-79-2
5g,25g,100g
焦磷酰氯
Diphosphoryl chloride
97%
13498-14-1
5g,25g,100g,500g
5-氯水杨基-N,N-二异丙基亚磷酰胺
5-Chlorosaligenyl-N,N-diisopropylphosphoramidite
>95.0%(HPLC)
1620086-77-2
200mg,1g
2-氯-1,3,2-二氧磷杂环戊烷
2-Chloro-1,3,2-dioxaphospholane
>97.0%(GC)
822-39-9
5g,25g,100g
N,N-二异丙基亚磷酰胺二叔丁酯
Di-tert-butyl N,N-diisopropylphosphoramidite
≥95.0%
137348-86-8
1g,5g
二苄基 N,N-二异丙基亚磷酰胺
Dibenzyl N,N-Diisopropylphosphoramidite
98%
108549-23-1
1g,5g,25g,100g
缩合剂
产品号 |
中文名称 |
英文名称 |
级别 |
Cas |
规格 |
3-硝基-1,2,4-三唑
3-Nitro-1,2,4-triazole
≥98.0%(HPLC)
24807-55-4
1g,5g,25g,100g
均三甲苯基磺酰氯
2-Mesitylenesulfonyl chloride
99%
773-64-8
5g,25g,100g,500g
N,N'-羰基二(1,2,4-三氮唑)
1,1'-Carbonyl-di-(1,2,4-triazole)
96%
41864-22-6
5g,25g,100g
2,4-二磺酰氯基均三甲苯
2,4-Mesitylenedisulfonyl Dichloride
≥98.0%
68985-08-0
5g,25g
特戊酰氯
Trimethylacetyl chloride
98%
3282-30-2
25ml,100ml,500ml,2.5L,10L
5-苄硫基四氮唑
5-(Benzylthio)-1H-tetrazole
98.50%
21871-47-6
5g,25g,100g
N,N'-二环己基碳二亚胺(DCC)
N,N′-Dicyclohexylcarbodiimide
99.00%
538-75-0
25g,100g,500g,1kg,2.5kg,10kg
1-(氰甲基)哌啶四氟硼酸盐
1-(Cyanomethyl)piperidinium Tetrafluoroborate
>98.0%(N)
434937-12-9
1g,5g
1-(2,4,6-三异丙基苯基磺酰)咪唑
1-(2,4,6-Triisopropylbenzenesulfonyl)imidazole
98%
50257-40-4
1g,5g,10g,25g
4,5-二氰基咪唑
4,5-Dicyanoimidazole
98%
1122-28-7
5g,25g,100g
4,5-二氰基咪唑
4,5-Dicyanoimidazole
99%
1122-28-7
5g,25g,100g
2,4,5,6-四甲基苯二磺酰二氯
2,4,5,6-Tetramethylbenzenedisulfonyl Dichloride
>98.0%(T)
97997-76-7
1g,5g
1-(2,4,6-三异丙基苯磺酰)-1,2,4-三唑
1-(2,4,6-Triisopropylphenylsulfonyl)-1,2,4-triazole
>98.0%(HPLC)
54230-60-3
1g,5g,25g
1-对甲基苯磺酰咪唑
1-(p-Toluenesulfonyl)imidazole
99%
2232-08-8
5g,25g,100g,500g
2,4,6-三异丙基苯磺酰氯
2,4,6-Triisopropylbenzenesulfonyl chloride
97%
6553-96-4
5g,25g,100g,500g
N,N'-二环己基-4-吗啉脒
N,N′-Dicyclohexyl-4-morpholinecarboxamidine
98%
4975-73-9
5g,25g,100g,500g
1,2,4-三氮唑
1,2,4-Triazole
99%
288-88-0
25g,100g,250g,500g
5-乙硫基四氮唑
5-(Ethylthio)-1H-tetrazole
98%
89797-68-2
1g,5g,25g,100g
羟基保护剂，氨基保护剂
产品号 |
中文名称 |
英文名称 |
级别 |
Cas |
规格 |
异丁酰氯
Isobutyryl Chloride
98%
79-30-1
25ml,100ml,500ml,2.5L
六甲基二硅胺烷
Hexamethyl disilylamine
AR,98%
999-97-3
100ml,500ml,2.5L,10L
二叔丁基二氯硅烷
Di-tert-butyldichlorosilane
>95.0%(GC)
18395-90-9
1g,5g
2-硝基溴化苄
2-Nitrobenzyl bromide
97%
3958-60-9
5g,25g,100g
苯氧基乙酰氯
Phenoxyacetyl Chloride
>98.0%(GC)
701-99-5
25g,100g,500g
1,3-苯并二硫代吡咯四氟化硼盐[羟基保护剂]
1,3-Benzodithiolylium Tetrafluoroborate [Hydroxyl-Protecting Agent]
>96.0%(T)
57842-27-0
1g,5g
N,O-双(三甲基硅烷基)乙酰胺
N,O-Bis(trimethylsilyl)acetamide
95%
10416-59-8
25ml,100ml,500ml,5L
N,O-双(三甲基硅烷基)乙酰胺
TMS-BA
用于GC衍生化
10416-59-8
5ml,25ml
乙酰氯
Acetyl chloride
99%,无色
75-36-5
500ml,2.5L
乙酰氯
Acetyl chloride
AR,98%
75-36-5
100ml,500ml,12*500ml,2.5L,10L
2-氯代三苯甲基氯
2-Chlorotrityl Chloride
≥97.0%
42074-68-0
25g,100g,500g,2.5kg
特戊酰氯
Trimethylacetyl chloride
98%
3282-30-2
25ml,100ml,500ml,2.5L,10L
N,N-二甲基甲酰胺二甲缩醛
DMF-DMA
98%,用于酯化
4637-24-5
25g,100g,500g
N,N-二甲基甲酰胺二甲缩醛
N,N-Dimethylformamide Dimethyl Acetal
97%
4637-24-5
25ml,100ml,500ml
三苯基氯甲烷
Trityl Chloride
97%
76-83-5
25g,100g,250g,500g,2.5kg
4-氯苯氧基乙酰氯
4-Chlorophenoxyacetyl Chloride
>98.0%(T)
4122-68-3
5g,10g,25g,100g,500g
2,4,6-三异丙基苯磺酰氯
2,4,6-Triisopropylbenzenesulfonyl chloride
97%
6553-96-4
5g,25g,100g,500g
4,4'-二甲氧基三苯基氯甲烷
4,4′-Dimethoxytrityl chloride
>97.0%
40615-36-9
5g,25g,100g,500g
二叔丁基硅基双(三氟甲烷磺酸)
Di-tert-butylsilyl bis(trifluoromethanesulfonate)
97%
85272-31-7
1g,5g,25g
叔丁基二苯基氯硅烷
tert-Butyldiphenylchlorosilane
98%
58479-61-1
10g,25g,100g,250g
叔丁基二甲基氯硅烷
tert-Butyldimethylsilyl chloride
97%
18162-48-6
25g,100g,250g,500g
4,4',4''-三(苯酰氧基)三苯甲基溴[羟基保护剂]
4,4',4''-Tris(benzoyloxy)trityl Bromide [Hydroxyl Protecting Agent]
>97.0%(T)
86610-66-4
1g,5g,25g
1,3-二氯四甲基二硅氧烷
1,3-Dichloro-1,1,3,3-tetramethyldisiloxane
95%
2401-73-2
5g,25g,100g
1,3二氯-1,1,3,3-四异丙基二硅氧烷
1,3-Dichloro-1,1,3,3-tetraisopropyldisiloxane
≥97%
69304-37-6
1g,5g,25g,100g
4-甲氧基苯甲酰氯
4-Methoxybenzoyl chloride
97%
100-07-2
25g,100g,500g
苯甲酰氯
Benzoyl chloride
ACS, ≥99.0% (T)
98-88-4
500g
三甲基氯硅烷(TMCS)
Chlorotrimethylsilane
≥99.0%(GC)
75-77-4
100ml,250ml,500ml,2.5L,10L
三甲基氯硅烷(TMCS)
Chlorotrimethylsilane
>98.0%(GC)
75-77-4
100ml,250ml,500ml,2.5L,10L
三氟甲磺酸三甲基硅酯
Trimethylsilyl trifluoromethanesulfonate
98.00%
27607-77-8
5g,10g,25g,50g,100g,250g,500g
4-甲氧基氯化三苯甲烷
4-Monomethoxytrityl chloride
97%
14470-28-1
5g,10g,25g,100g,250g
苯甲酸酐
Benzoic anhydride
98%
93-97-0
25g,100g,500g
脱保护剂
产品号 |
中文名称 |
英文名称 |
级别 |
Cas |
规格 |
三氟乙酸（TFA）
Trifluoroacetic acid
AR,99.0%
76-05-1
100g,500g,2.5kg
六氟异丙醇
1,1,1,3,3,3-Hexafluoro-2-propanol
99.50%
920-66-1
25g,100g,250g,500g,2.5kg