测量环AMP（cAMP）信号传导动力学，筛选Gs活性的GPCR，以及使用活细胞、荧光、cADDis cAMP测定的更多功能。将cADDis传感器添加到您的细胞中，并在第二天在自动酶标仪或荧光成像系统上测量稳健的信号。增加的绿色荧光指示cAMP增加。该检测试剂在BacMam中进行遗传编码和包装，可在大多数细胞类型中进行有效转导-包括在HEK 293细胞中的均匀和接近100%的效率以及在许多原代或iPSC衍生细胞中的表达。

Montana Molecular-GPCR检测试剂盒包括cAMP传感器BacMam、500 mM丁酸钠水溶液以及阳性对照受体和激动剂（B2 AR BacMam和10 mM异丙肾上腺素HCl溶液）。30 mL和60 mL的尺寸也可用。其他Montana分子工具，包括BacMam包装的GPCR或红色荧光DAG和钙传感器，可以很容易地与该测定共表达。

GPCR检测套装（#U0200G）中包含的材料：

绿色增强型cADDis cAMP传感器BacMam

500 mM的丁酸钠，溶于H2O

带有核红标记的β2肾上腺素受体BacMam

10 mM的异丙肾上腺素，溶于10 mM的盐酸中

GPCR检测参考：

Borealis Arrestin Assay References：

H. Schiff, et al. β-arrestin-biased proteinase-activated receptor-2 antagonist C781 limits allergen-induced airway hyperresponsiveness and inflammation. British Journal of Pharmacology. June 2022.

cADDis cAMP Assay References：

B. Polacco, et al. Profiling the proximal proteome of the activated μ-opioid receptor. nature chemical biology. March 2024.

DAG Assay References：

X. Chen, et al. A PACAP-activated network for secretion requires coordination of Ca2+ influx and Ca2+ mobilization. bioRxiv. January 2024.

PIP2 Assay References：

L. Brueggemann, et al. Structural Determinants of Kv7.5 Potassium Channels that Confer Changes in Phosphatidylinositol 4,5-Biphosphate (PIP2) Affinity and Signaling Sensitivity in Smooth Muscle Cells. Molecular Pharmacology. March 2020.

GECO Calcium Assay References：

C. Amos, et al. Membrane lipids couple synaptotagmin to SNARE-mediated granule fusion in insulin-secreting cells. Molecular Biology of the Cell. December 2023.

GPCR检测相关产品：

#U0600R Red GECO Ca2+ Assay Kit

#D0400R Red PIP2 Assay Kit

#U0601R Mitochondrial-targeted Red GECO Assay Kit

#D0241G Green Membrane-Targeted fS15 cADDis cAMP

https://www.amyjet.com/products/MTA-U0200G-010.shtml