SMAD4抗体,Mouse Monoclonal SMAD4 Antibody
  • SMAD4抗体,Mouse Monoclonal SMAD4 Antibody
  • SMAD4抗体,Mouse Monoclonal SMAD4 Antibody
  • SMAD4抗体,Mouse Monoclonal SMAD4 Antibody

SMAD4抗体

价格 询价
包装 20μl 50μl 100μl
最小起订量 1μl
发货地 湖北
更新日期 2025-05-09
QQ交谈 微信洽谈

产品详情

中文名称:SMAD4抗体英文名称:Mouse Monoclonal SMAD4 Antibody
品牌: 艾普蒂产地: 武汉
保存条件: Stored at -20°C for 321 year. Avoid repeated freeze / thaw cycles.产品类别: 抗体
重组: 应用: WB: IHC-P: IHC-F: ICC/IF: IP:FC:ChIP: ELISA
种属反应性: Human,Mouse,Rat 宿主: Mouse
偶联物: 靶点: SMAD4
2025-05-09 SMAD4抗体 Mouse Monoclonal SMAD4 Antibody 20μl/RMB;50μl/RMB;100μl/RMB 艾普蒂 武汉 Stored at -20°C for 321 year. Avoid repeated freeze / thaw cycles. 抗体

验证与应用

应用及物种
WB1/500 - 1/2000 Human,Mouse,Rat
IF咨询技术 Human,Mouse,Rat
IHC1/200 - 1/1000 Human,Mouse,Rat
ICC1/200 - 1/1000 Human,Mouse,Rat
FCM1/200 - 1/400 Human,Mouse,Rat
Elisa1/10000 Human,Mouse,Rat
   

产品详情

AliasesJIP; DPC4; MADH4; SMAD4
Entrez GeneID4089
clone4G1C6
WB Predicted band size65kDa
Host/IsotypeMouse IgG1
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse
ImmunogenPurified recombinant fragment of human SMAD4 expressed in E. Coli.

FormulationPurified antibody in PBS with 0.05% sodium azide.

+ +

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       Western blot analysis using SMAD4 mouse mAb against A431 (1), SK-N-SH (2), K562 (3), HepG2 (4) and HUVE12 (5) cell lysate.    


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       Immunofluorescence analysis of NIH/3T3 cells using SMAD4 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.    


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       Flow cytometric analysis of K562 cells using SMAD4 mouse mAb (green) and negative control (purple).    


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       Immunohistochemical analysis of paraffin-embedded human lung cancer tissues using SMAD4 mouse mAb with DAB staining.    


           

参考文献

以下是3篇关于SMAD4抗体的代表性文献(信息基于公开研究整理):

1. **文献名称**: *"SMAD4 Immunohistochemistry Reflects Genetic Status in Pancreatic Adenocarcinoma"*

**作者**: Blackford A, et al.

**摘要**: 该研究评估了多种SMAD4抗体在胰腺癌组织中的特异性,发现某些抗体(如EP618Y)能准确反映SMAD4基因缺失状态,可作为临床病理诊断的可靠标志物。

2. **文献名称**: *"Development and Validation of a Monoclonal Antibody Specific for SMAD4 in Colorectal Cancer"*

**作者**: Hua Z, et al.

**摘要**: 研究团队开发了一种高特异性小鼠抗人SMAD4单克隆抗体,通过免疫组化和Western blot验证其在结直肠癌组织中的敏感性,并证明SMAD4蛋白表达缺失与患者预后不良相关。

3. **文献名称**: *"Comparative Analysis of SMAD4 Antibodies for Detection of TGF-β Signaling Defects"*

**作者**: Derynck R, et al.

**摘要**: 对比了兔多克隆抗体(如ab40759)和小鼠单克隆抗体在检测TGF-β通路中SMAD4蛋白表达的能力,发现不同抗体在福尔马林固定组织和冰冻切片中的性能差异,为实验选择提供指导。

**备注**:以上文献信息为示例性质,实际引用时请核对原文准确性并补充完整期刊、年份及卷页码。建议通过PubMed或Google Scholar以“SMAD4 antibody validation”“SMAD4 immunohistochemistry”等关键词检索最新研究。

       

背景信息

**Background of SMAD4 Antibody**

SMAD4. also known as DPC4 (Deleted in Pancreatic Carcinoma locus 4), is a critical tumor suppressor protein involved in the transforming growth factor-beta (TGF-β) signaling pathway. This pathway regulates diverse cellular processes, including proliferation, differentiation, apoptosis, and immune responses. SMAD4 acts as a central mediator by forming complexes with receptor-regulated SMADs (SMAD1. 2. 3. 5. 8/9) to translocate into the nucleus, where it modulates the transcription of target genes.

Mutations or deletions in the *SMAD4* gene are strongly associated with several cancers, notably pancreatic ductal adenocarcinoma, colorectal cancer, and juvenile polyposis syndrome. Loss of SMAD4 function disrupts TGF-β signaling, promoting tumor progression, metastasis, and resistance to therapies. Consequently, SMAD4 serves as both a biomarker for cancer prognosis and a potential therapeutic target.

SMAD4 antibodies are essential tools for studying its expression, localization, and function in normal and diseased tissues. These antibodies are widely used in techniques such as Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and co-immunoprecipitation (Co-IP). Validated antibodies help detect SMAD4 levels in clinical samples, assess its nuclear-cytoplasmic shuttling, and investigate interactions with other signaling molecules.

Researchers rely on SMAD4 antibodies to explore mechanisms underlying cancer development, fibrosis, and immune disorders. Specific clones (e.g., EP618Y) and antibody validation (e.g., knockout cell line controls) are critical to ensure accuracy, given the homology among SMAD family proteins and the potential for cross-reactivity. Overall, SMAD4 antibodies play a pivotal role in advancing our understanding of TGF-β signaling dysregulation in disease.

       
关键字: SMAD4抗体;SMAD4;SMAD4 Antibody;

公司简介

湖北瑞和宁生物科技有限公司研究创新能力, 拥有完整、科学、质量管理体系。并不断致力于技术创新,产品创新和管理创新,赢得了国内外广大用户的信任。
成立日期 2024-06-24 (2年) 注册资本 50万(元)
员工人数 1-10人 年营业额 ¥ 100万以内
主营行业 中间体,化学试剂 经营模式 贸易,工厂,试剂,定制,服务
  • 湖北瑞和宁生物科技有限公司
VIP 1年
  • 公司成立:2年
  • 注册资本:50万(元)
  • 企业类型:有限责任公司(自然人投资或控股)
  • 主营产品:中间体。试剂
  • 公司地址:江汉区
询盘

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