The SLIT3 antibody is a tool used to detect and study SLIT3. a secreted glycoprotein belonging to the SLIT family (SLIT1-3). SLIT proteins are evolutionarily conserved ligands known for their roles in axon guidance, neuronal migration, and cellular signaling by interacting with Roundabout (ROBO) receptors. SLIT3. encoded by the *SLIT3* gene on human chromosome 5q35. is involved in diverse biological processes, including angiogenesis, tissue remodeling, and organ development. Unlike SLIT1 and SLIT2. which are primarily neural, SLIT3 exhibits broader expression in non-neural tissues like the heart, kidneys, and skeletal system.
Antibodies targeting SLIT3 enable researchers to investigate its expression patterns, localization, and interactions in physiological and pathological contexts. They are widely used in techniques like immunohistochemistry, Western blotting, and immunofluorescence. SLIT3 has been implicated in diseases such as cancer, where it may act as a tumor suppressor or promoter depending on the context, and cardiovascular disorders, where it regulates vascular smooth muscle cell behavior. Its role in bone metabolism and fibrosis is also under active study.
Commercial SLIT3 antibodies vary in specificity (monoclonal vs. polyclonal) and host species (e.g., rabbit, mouse). Validation via knockout controls or peptide blocking is critical to ensure reliability. Research using these antibodies continues to unravel SLIT3’s complex functions, offering potential therapeutic insights for conditions like metastasis, atherosclerosis, and tissue scarring.