SRGAP1 (Slit-Robo GTPase-Activating Protein 1) is a member of the evolutionarily conserved SRGAP family, known for its role in regulating neuronal development and synaptic plasticity. It functions as a GTPase-activating protein (GAP) for small GTPases like Cdc42. influencing cytoskeletal dynamics critical for axon guidance, dendritic branching, and neuronal migration. Structurally, SRGAP1 contains an F-BAR domain (mediating membrane curvature sensing), an SH3 domain (facilitating protein-protein interactions), and a GAP domain (enzymatic activity). Its interactions with Slit-Robo signaling pathways highlight its importance in neurodevelopmental processes.
Antibodies targeting SRGAP1 are essential tools for studying its expression, localization, and function in cellular and tissue contexts. These antibodies are widely used in techniques such as Western blotting, immunohistochemistry, and immunofluorescence to investigate SRGAP1's role in brain development and disease. Research links SRGAP1 dysregulation to neurodevelopmental disorders, intellectual disabilities, and psychiatric conditions, underscoring its clinical relevance.
Validated SRGAP1 antibodies are commercially available from suppliers like Abcam, Cell Signaling Technology, and Santa Cruz Biotechnology. Specificity is confirmed through knockout controls or siRNA knockdown experiments. Recent studies also explore SRGAP1's interplay with paralogs (e.g., SRGAP2/SRGAP3) in neuronal evolution and disease mechanisms, making these antibodies pivotal for advancing neuroscience and therapeutic discovery.