The heterogeneous nuclear ribonucleoprotein C (HNRNPC) is a member of the hnRNP family, which plays critical roles in RNA metabolism, including pre-mRNA splicing, RNA transport, stability, and translation. HNRNPC primarily binds to uridine-rich RNA sequences, influencing mRNA processing and gene expression regulation. It is ubiquitously expressed and localizes to the nucleus, where it associates with nascent RNA transcripts. Dysregulation of HNRNPC has been linked to various diseases, including cancers, neurodegenerative disorders, and viral infections, due to its role in post-transcriptional regulation and stress response pathways.
HNRNPC-specific antibodies are essential tools for studying its molecular functions, interactions, and expression patterns. These antibodies are widely used in techniques like Western blotting, immunoprecipitation (IP), immunofluorescence (IF), and chromatin immunoprecipitation (ChIP) to investigate HNRNPC's involvement in RNA-protein complexes, DNA damage responses, and alternative splicing mechanisms. Research utilizing HNRNPC antibodies has revealed its contribution to cancer progression, such as promoting metastasis through alternative splicing of oncogenic transcripts, and its role in neurological conditions like amyotrophic lateral sclerosis (ALS). Additionally, studies highlight its interaction with viral RNAs, impacting viral replication cycles. Validated antibodies against HNRNPC are crucial for ensuring specificity in these applications, aiding advancements in understanding RNA biology and disease mechanisms.