DCTN4抗体—艾普蒂

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     Western blot analysis using DCTN4 mouse mAb against Raw264.7 (1) and NIH3T3 (2) cell lysate.    

  
  

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     Immunofluorescence analysis of HepG2 cells using DCTN4 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.    

  
  

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     Flow cytometric analysis of A549 cells using DCTN4 mouse mAb (green) and negative control (red).    

  
  

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     Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using DCTN4 mouse mAb with DAB staining.    

  
  

The DCTN4 antibody targets dynactin subunit 4 (DCTN4), a key component of the dynactin complex, which works alongside cytoplasmic dynein to regulate microtubule-based intracellular transport. DCTN4. also known as p62. stabilizes the dynactin structure and facilitates interactions between dynein, cargo adaptors, and organelles. This protein is critical for maintaining cellular processes like vesicle trafficking, mitotic spindle orientation, and neuronal axonal transport. Dysregulation of dynactin function is implicated in neurodegenerative diseases, including amyotrophic lateral sclerosis (ALS) and Perry syndrome, as well as cancer metastasis.

DCTN4 antibodies are primarily utilized in research to study the expression, localization, and functional roles of DCTN4 in both normal and pathological conditions. They are essential tools for techniques such as Western blotting, immunohistochemistry, and immunofluorescence, enabling visualization of DCTN4 distribution in tissues or cultured cells. Studies using these antibodies have revealed DCTN4's involvement in cargo-binding specificity and its potential as a biomarker for diseases linked to dynein-dynactin dysfunction. Additionally, DCTN4 antibodies aid in exploring therapeutic strategies targeting microtubule-dependent transport mechanisms, offering insights into disease mechanisms and drug development. Their specificity and reliability make them valuable for dissecting dynactin-related cellular pathways.