SF3B3抗体;SF3B3 Antibody—艾普蒂

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     Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)    

  
  

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     Immunohistochemical analysis of paraffin-embedded human brain tissues using GZMB mouse mAb with DAB staining.    

  
  

The SF3B3 (Splicing Factor 3B Subunit 3) antibody is a tool used to detect and study the SF3B3 protein, a core component of the spliceosome complex responsible for pre-mRNA splicing. SF3B3 is part of the SF3b complex within U2 small nuclear ribonucleoprotein (snRNP), playing a critical role in recognizing branch site sequences during RNA splicing. This protein is essential for maintaining genomic stability and regulating gene expression.

Antibodies targeting SF3B3 are widely utilized in research to investigate spliceosome assembly, RNA processing mechanisms, and their dysregulation in diseases. Notably, SF3B3 mutations or aberrant expression have been linked to cancers, including hematologic malignancies and solid tumors, making it a potential biomarker or therapeutic target. These antibodies are employed in techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and co-immunoprecipitation (Co-IP) to analyze SF3B3 expression, localization, and interactions in cellular models or clinical samples.

Commercial SF3B3 antibodies are typically validated for specificity and sensitivity across species (e.g., human, mouse, rat). Researchers often verify results using siRNA knockdown or CRISPR-based gene editing to confirm protein identity. Its study contributes to understanding splicing-related pathologies and developing targeted therapies for splicing factor-mutated cancers.