ARHGEF6 antibody is a tool used to detect ARHGEF6. a protein encoded by the ARHGEF6 gene, which belongs to the Rho guanine nucleotide exchange factor (GEF) family. Rho GEFs regulate Rho GTPases—key signaling molecules controlling cytoskeletal reorganization, cell migration, and adhesion. ARHGEF6. also known as α-Pix or Cool-2. specifically activates Rac1 and Cdc42 by catalyzing GDP-to-GTP exchange. It contains modular domains, including a SH3 domain that facilitates interactions with signaling partners like PAK kinases, linking it to pathways regulating cell motility and polarity.
Research using ARHGEF6 antibodies has elucidated its role in neural development, immune cell trafficking, and cancer metastasis. In neurons, ARHGEF6 governs dendritic spine formation and synaptic plasticity, while in immune cells, it modulates chemokine-directed migration. Dysregulation of ARHGEF6 is implicated in X-linked intellectual disability and tumor invasiveness, making it a focus in neurodevelopmental and oncology studies.
ARHGEF6 antibodies are typically validated in applications such as Western blotting, immunofluorescence, and immunoprecipitation to assess protein expression, localization, and interactions. Specificity is confirmed via knockout controls or siRNA knockdown. These reagents are critical for exploring ARHGEF6’s molecular mechanisms, including its cross-talk with integrin signaling or growth factor receptors, and its potential as a therapeutic target in diseases involving aberrant cell motility or synaptic dysfunction.