TBX3抗体;TBX3 Antibody—艾普蒂

+ +

•  

   

     Gel: 6%SDS-PAGE, Lysate: 40 μg, Lane: HepG2 cell lysate, Primary antibody: P12632(TBX3 Antibody) at dilution 1/800, Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution, Exposure time: 2 minutes    

  
  

•  

   

     The image is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using P12632(TBX3 Antibody) at dilution 1/35. (Original magnification: ×200)    

  
  

•  

   

     The image is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using P12632(TBX3 Antibody) at dilution 1/35. (Original magnification: ×200)    

  
  

The TBX3 antibody is a crucial tool in studying the TBX3 protein, a member of the T-box transcription factor family. TBX3 plays pivotal roles in embryonic development, particularly in limb, heart, and organogenesis. It shares functional overlap with TBX5. and both regulate gene networks involved in cell differentiation, proliferation, and tissue patterning. Notably, TBX3 is implicated in suppressing the Hippo signaling pathway, influencing organ size and tumorigenesis. Dysregulation of TBX3 expression is linked to developmental disorders like ulnar-mammary syndrome (caused by TBX3 mutations) and cancers, including breast cancer, melanoma, and hepatocellular carcinoma, where it often promotes metastasis and chemoresistance.

TBX3 antibodies are primarily used in research to detect and quantify TBX3 protein levels via techniques like Western blotting, immunohistochemistry, and immunofluorescence. These antibodies aid in elucidating TBX3's spatial-temporal expression, post-translational modifications, and interactions with signaling pathways. Commercial TBX3 antibodies are typically raised against specific epitopes (e.g., human TBX3 amino acid regions) and vary in clonality (monoclonal/polyclonal), host species, and validation standards. Challenges include ensuring specificity due to homology within the T-box family and cross-reactivity risks. Recent studies leverage TBX3 antibodies to explore its dual role as a tumor suppressor or oncogene, depending on context, and its potential as a therapeutic target. Validation using knockout controls or siRNA is critical for reliable experimental outcomes.