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HSC70 抗体
品牌：MedChemExpress (MCE)
货号：HY-P85704
存储条件：-20°C保存1年。避免重复冻结/解冻循环。
运输条件：用蓝冰运输。
产品活性：HSC70 Antibody (YA5396) 是一种小鼠来源、无偶联标记、抗 HSC70 的单克隆抗体。它可用于人、小鼠、大鼠背景下的 IHC-P、WB 实验。
研究背景：HSC70 是一种分子伴侣，参与多种细胞过程，包括保护蛋白质组免受应激、新合成多肽的折叠和运输、伴侣介导的自噬 (chaperone-mediated autophagy)、错误折叠蛋白的激活降解、蛋白质复合物的形成和解离以及抗原呈递。它在蛋白质质量控制系统中发挥关键作用，确保蛋白质的正确折叠、错误折叠蛋白的重新折叠以及控制蛋白质的后续降解靶向。这一过程通过 ATP 结合、ATP 水解和 ADP 释放的循环实现，并由共伴侣 (co-chaperones) 介导。HSC70 对多肽的亲和力受其核苷酸结合状态调控：ATP 结合状态下对底物蛋白亲和力低，而 ATP 水解为 ADP 后构象变化会增加亲和力。其共伴侣分为三类：J 结构域共伴侣 HSP40s（刺激 HSC70 的 ATPase 活性）、核苷酸交换因子 (NEF) 如 BAG1/2/3（促进 ADP 到 ATP 的转换从而释放底物）以及 TPR 结构域伴侣如 HOPX 和 STUB1。此外，HSC70 在线粒体导入中起关键作用，将前蛋白递送至线粒体导入受体 TOMM70；抑制转录激活；作为 PRP19-CDC5L 复合物的组分参与剪接体激活；结合细菌脂多糖 (LPS) 介导炎症反应；在伴侣介导的自噬中识别含 -KFERQ 基序的底物并引导至溶酶体降解；与 LAMP2 协作促进胞质抗原的 MHC II 类呈递；参与 ER 相关降解 (ERAD) 通路；并通过与 DNAJC6 相互作用被招募至网格蛋白包被囊泡，激活 ATPase 活性以脱去网格蛋白涂层 (by similar)。
宿主：Mouse
反应物种：Human, Mouse, Rat
推荐稀释比例：IHC1:50-200; WB: 1:1000-2000 Immunohistochemical analysis of paraffin-embedded mouse colon tissue using HSC70 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. Immunohistochemical analysis of paraffin-embedded mouse colon tissue using HSC70 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
基因 ID：3312
同用名：Heat shock cognate 71 kDa protein; Heat shock 70 kDa protein 8;
克隆性：Monoclonal
偶联：Non-conjugated
修饰：Unmodified
蛋白数据库：P11142
详情参考：www.medchemexpress.cn/antibody/hsc70-antibody-ya5396.html