SC5502-5mg
SMER28 (Autophagy增强剂)
5mg
316.00元
化学信息:
化学名
6-bromo-N-prop-2-enylquinazolin-4-amine
溶剂/溶解度
Water<1mg/ml; DMSO52mg/ml; Ethanol52mg/ml
溶液配制
5mg加入1.89ml DMSO,或者每2.64mg加入1ml DMSO,配制成10mM溶液。SC5502-10mM用DMSO配制。
生物信息:
产品描述
SMER28 is a small-molecule enhancer (SMER) of autophagy, inducing autophagy independently of rapamycin in mammalian cells.
体外研究
SMER28 is a positive regulator of autophagy in a mechanism independent from the mTOR pathway. It increases autophagosome synthesis and enhances the clearance of model autophagy substrates such as A53T a-synuclein and mutant huntingtin fragments. A marked decrease in Aβ/APP-CTF levels and enhancement for degradation of Aβ/APP-CTF via autophagy is also induced by SMER28. APP-CTF and LC3-II are cocompartmentalized on SMER28 treatment.
相关实验数据(此数据来自于公开文献,碧云天并不保证其有效性):
细胞系
MEF cells overexpressing βCTF/ N2a-APP cells/ Rat primary neuronal cultures
浓度
10μM in MEF cells/0-50μM in N2a-APP cells and rat primary neuronal cultures
方法
MEF cells overexpressing βCTF are treated with autophagy-enhancing compounds (10μM) for 16 h. Cell lysates are analyzed by SDS-PAGE and Western blotting using anti-βCTF (6E10) and anti-tubulin antibodies.
参考文献:
1. Tian Y, et al. FASEB J. 2011, 25(6):1934-1942.
包装清单:
SC5502-10mM
SMER28 (Autophagy增强剂)
10mM×0.2ml
SC5502-5mg
SMER28 (Autophagy增强剂)
5mg
SC5502-25mg
SMER28 (Autophagy增强剂)
25mg
保存条件:
-20℃保存,至少一年有效。5mg和25mg包装也可室温保存,至少6个月有效。如果溶于非DMSO溶剂,建议分装后-80℃保存,预计6个月内有效。
注意事项:
本产品对人体有刺激性,操作时请小心,并注意适当防护以避免直接接触人体或吸入体内。
本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
为了您的安全和健康,请穿实验服并戴一次性手套操作。