SF1159-25mg
SKF96365 (TRPC抑制剂)
25mg
1318.00元
化学信息：
化学名
1-[2-(4-methoxyphenyl)-2-[3-(4-methoxyphenyl)propoxy]ethyl]imidazole;hydrochloride
别名
SK and F 96365, SK and F-96365, SKF-96365, SKF 96365, SKF 96365 HYDROCHLORIDE
溶剂/溶解度
Water80mg/ml; DMSO80mg/ml; Ethanol80mg/ml
溶液配制
5mg加入1.24ml DMSO，或者每4.03mg加入1ml DMSO，配制成10mM溶液。SF1159-10mM用DMSO配制。
生物信息：
产品描述
SKF96365, originally identified as a blocker of receptor-mediated calcium entry, is widely used diagnostically, as a blocker of transient receptor potential canonical type (TRPC) channels.
体外研究
SKF-96365 exhibits protective activity against MPP+ injury in PC12 cells and significantly inhibits apoptotic cell death in PC12 cells after MPP+ administration. SKF-96365 does not exert effects on necrotic cell death induced by MPP+ insult in PC12 cells. Because of its non-selective activity, SKF-96365 has been demonstrated to have effects on multiple other Ca2+ channels: it not only blocks high-voltage-activated (HVA) Ca2+ channels at typically utilized test concentrations, but also potently inhibits low-voltage -activated (LVA) T-type Ca2+ channels in HEK293 cells. The exact effect of SKF-96365 on intracellular calcium homeostasis might dependent on cell types and experimental models used.
体内研究
SKF-96365 treatment inhibited the calcium/calmodulin-dependent protein kinase IIγ (CaMKIIγ)/AKT signaling cascade in vitro and in vivo.
相关实验数据(此数据来自于公开文献，碧云天并不保证其有效性)：
方法
To investigate whether SKF-96365 could protect PC12 cells from injury induced by MPP+ insult, cultured PC12 cells are pretreated with SKF-96365 in different concentrations (1μM, 10μM or 50μM) 30 min before MPP+ addition. The cells viability is measured 24h after MPP+ insult by using the cell proliferation reagent WST-1.
剂量
0.02μMol-0.2μMol/2μl/mouse
参考文献：
1. Singh A, et al. Br J Pharmacol. 2010, 160(6):1464-1475.
2. Chen T, et al. PLoS One. 2013, 8(1):e55601.
3. Yao H, et al. Cell Death Differ. 2009, 16(12):1681-1693.
4. Jing Z, et al. Cancer Lett. 2016, 372(2):226-38.
包装清单：
SF1159-10mM
SKF96365 (TRPC抑制剂)
10mM×0.2ml
SF1159-5mg
SKF96365 (TRPC抑制剂)
5mg
SF1159-25mg
SKF96365 (TRPC抑制剂)
25mg
保存条件：
-20℃保存，至少一年有效。5mg和25mg包装也可室温保存，至少6个月有效。如果溶于非DMSO溶剂，建议分装后-80℃保存，预计6个月内有效。
注意事项：
本产品对人体有刺激性，操作时请小心，并注意适当防护以避免直接接触人体或吸入体内。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。