SF1136-25mg
SCR7 (DNA ligase IV抑制剂)
25mg
1600.00元
化学信息：
化学名
5-(benzylideneamino)-6-[(E)-benzylideneamino]-2-sulfanylidene-1H-pyrimidin-4-one
别名
CS-3903, AK174235, BC600700, QC-11823, X3557
溶剂/溶解度
Water<1mg/ml; DMSO45mg/ml; Ethanol<1mg/ml
溶液配制
5mg加入1.5ml DMSO，或者每3.34mg加入1ml DMSO，配制成10mM溶液。SF1136-10mM用DMSO配制。
生物信息：
产品描述
SCR7 is a molecule that inhibits joining of DSBs in cell-free repair system; inhibits NHEJ (nonhomologous end-joining) in a Ligase IV-dependent manner within cells, and activates the intrinsic apoptotic pathway.
靶点
Ligase IV inhibitor
NHEJ inhibitor
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体外研究
SCR7 does not induce DSBs directly to the genome and is Ligase IV dependent. Besides, upon incubation of oligomeric dsDNA or supercoiled plasmid DNA with increasing concentrations of SCR7, there was no evidence for DNA breaks. Accumulation of DSBs leads to cell death upon SCR7 treatment with a dose-dependent decrease in cell proliferation of MCF7, A549, and HeLa with an IC50 of 40, 34 and 44μM, respectively, which was further confirmed by DIC imaging in MCF7. T47D, A2780 and HT1080 were also sensitive to SCR7, with an IC50 of 8.5, 120, and 10μM, respectively. SCR7 encapsulated micelles (ES) were also characterized by small-angle neutron scattering (SANS). Encapsulated SCR7 treatment resulted in accumulation of DNA breaks within the cells, resulting in cell cycle arrest at G1 phase and activation of apoptosis.
体内研究
SCR7 treatment (10mg/kg, six doses) significantly reduced breast adenocarcinoma-induced tumor in mice. Untreated tumor animals survived only for 52 days, whereas treated animals exhibited ～4-fold increase in lifespan. Treatment with SCR7 resulted in regression of tumors with no obvious adverse effects. In addition, HPLC analysis of serum following administration of SCR7 into mice (20mg/kg) showed bioavailability of 114μg/ml and a t1/2 of 1hr.
相关实验数据(此数据来自于公开文献，碧云天并不保证其有效性)：
参考文献：
1. Srivastava M, et al. Cell. 2012 Dec 21, 151(7), 1474-87.
包装清单：
SF1136-10mM
SCR7 (DNA ligase IV抑制剂)
10mM×0.2ml
SF1136-5mg
SCR7 (DNA ligase IV抑制剂)
5mg
SF1136-25mg
SCR7 (DNA ligase IV抑制剂)
25mg
保存条件：
-20℃保存，至少一年有效。5mg和25mg包装也可室温保存，至少6个月有效。如果溶于非DMSO溶剂，建议分装后-80℃保存，预计6个月内有效。
注意事项：
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。